| 平菇多糖提取方法的比较 |
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| 引用本文： | 张敏,陈瑞战,陈瑞平,刘志强,孟繁磊,李元,董航.平菇多糖提取方法的比较[J].吉林大学学报(理学版),2011,49(6):1141-1149. |
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| 作者姓名： | 张敏 陈瑞战 陈瑞平 刘志强 孟繁磊 李元 董航 |
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| 作者单位： | 1. 长春师范学院 化学学院, 长春 130032； 2. 中国科学院 长春应用化学研究所质谱中心, 长春 130022；3. 吉林省农业科学院, 长春 130033 |
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| 基金项目： | 吉林省自然科学基金(批准号:201015106) |
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| 摘 要： | 以平菇为原料, 分别采用破碎、 超声波和超声辅助复合酶3种提取方法提取平菇水溶性多糖. 正交优化结果表明： 提取温度90 ℃, 剪切速率11 350 r/min, 提取时间20 min, 提取3次, 破碎法提取多糖得率为11.20%； 提取温度60 ℃, 超声功率450 W, 提取时间60 min, 提取3次, 超声法提取多糖得率为9.75%； 提取温度30 ℃, 超声功率350 W, 提取时间60 min, 溶液pH=5, 超声辅助复合酶法提取多糖得率为12.05%. 综合考虑提取得率、 节能环保、 操作方法等因素, 选用破碎法提取平菇多糖较理想.
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| 关 键 词： | 平菇多糖 破碎提取 超声波提取 超声辅助复合酶 正交设计试验 |
| 收稿时间： | 2011-02-20 |
Comparison among Extraction Technologies of Polysaccharide from Pleurotus ostreatus |
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| ZHANG Min,CHEN Rui-zhan,CHEN Rui-ping,LIU Zhi-qiang,MENG Fan-lei,LI Yuan,DONG Hang.Comparison among Extraction Technologies of Polysaccharide from Pleurotus ostreatus[J].Journal of Jilin University: Sci Ed,2011,49(6):1141-1149. |
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| Authors: | ZHANG Min CHEN Rui-zhan CHEN Rui-ping LIU Zhi-qiang MENG Fan-lei LI Yuan DONG Hang |
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| Institution: | 1. College of Chemistry, Changchun Normal University, Changchun 130032, China|2. Center of Mass Spectrometry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;3. Jilin Academy of Agricultural Sciences, Changchun 130033, China |
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| Abstract: | The Pleurotus ostreatus were used as the raw material for extracting polysaccharide by means of smashing extractor, ultrasonic extraction, ultrasonic assisted complex enzyme extraction. The results indicate theoptimal extraction conditions of the smashing extractor were extraction temperature 90 ℃, extraction rate 11 350 r/min, extraction time 20 min, extraction 3 times, and the yield of polysaccharide extracted was 11.20%. The optimal extraction conditions of ultrasonic extraction were extraction temperature 60 ℃, extraction time 60 min, ultrasonic power 450 W, extraction 3 times, and the yield of polysaccharide extracted was 9.75%. The optimal extraction conditions of ultrasonic assisted complex enzyme extraction were extraction temperature 30 ℃, extraction time 60 min, ultrasonic power 350 W, extraction pH 5, the yield of polysaccharide extracted was 1205%. In comprehensive consideration of the yield of polysaccharide extracted, saving energy, simple operation, the smashing extractor was considered as an ideal method. |
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| Keywords: | Pleurotus ostreatus polysaccharide smashing extraction ultrasonic extraction ultrasonic-assisted complex enzyme orthogonal array design |
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