| 荧光法研究碱性介质中吡哆醛(VB6)与几种氨基酸及谷胱甘肽的相互作用 |
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| 引用本文： | 李萌,谢青季,姚守拙.荧光法研究碱性介质中吡哆醛(VB6)与几种氨基酸及谷胱甘肽的相互作用[J].湖南师范大学自然科学学报,2005,28(2):53-56. |
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| 作者姓名： | 李萌 谢青季 姚守拙 |
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| 作者单位： | 湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,中国,长沙,410081 |
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| 基金项目： | 国家自然科学基金资助项目(20275010);全国高校优秀青年教师教学科研奖励计划(教人司[2000]26号) |
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| 摘 要： | 研究了NaOH水溶液中甘氨酸、丙氨酸、半胱氨酸、胱氨酸、还原型谷胱甘肽和氧化型谷胱甘肽对吡哆醛的荧光猝灭行为．推导了荧光猝灭法研究物质间相互作用的一般方程，测算了吡哆醛与各氨基酸及谷胱甘肽作用的结合常数(K)与结合比(n)，并考察了氨基酸和谷胱甘肽电子光谱吸收对吡哆醛荧光测量的影响以及碱性介质中吡哆醛的荧光特性．测算和讨论了甘氨酸和吡哆醛结合反应的热力学函数．
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| 关 键 词： | 吡哆醛 荧光猝灭 相互作用 氨基酸 谷胱甘肽 |
| 文章编号： | 1000-2537(2005)02-0053-04 |
| 修稿时间： | 2004年11月4日 |
A Study on the Interactions of Pyridoxal (VB6) with Several Amino Acids and Glutathione in Aqueous NaOH by Fluorescence Spectrophotometry |
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| LI Meng,XIE Qing-ji,YAO Shou-zhuo.A Study on the Interactions of Pyridoxal (VB6) with Several Amino Acids and Glutathione in Aqueous NaOH by Fluorescence Spectrophotometry[J].Journal of Natural Science of Hunan Normal University,2005,28(2):53-56. |
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| Authors: | LI Meng XIE Qing-ji YAO Shou-zhuo |
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| Abstract: | The fluorescence quenching of pyridoxal in NaOH solution by glycine, alanine, cysteine, cystine, glutathione (reduced and oxidized forms) was investigated. A general equation for investigating interactions between molecules via fluorescence quenching method was deduced, and the binding constants (K) and binding ratios (n) of pyridoxal to these amino acids and glutathione were thus obtained. The fluorescent characteristics of pyridoxal in alkaline solutions and related UV- absorption influence from amino acids and glutathione were examined. The thermodynamic functions of the reaction of glycine with pyridoxal were measured and discussed. |
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| Keywords: | pyridoxal fluorescence quenching interaction amino acids glutathione |
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