| 聚丙烯酰胺凝胶电泳体系中蛋白质的荧光标记分析 |
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| 引用本文： | 王钒,陈仰三,董黎雾,庄庆祺. 聚丙烯酰胺凝胶电泳体系中蛋白质的荧光标记分析[J]. 华东理工大学学报(自然科学版), 1987, 0(5) |
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| 作者姓名： | 王钒 陈仰三 董黎雾 庄庆祺 |
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| 作者单位： | 华东化工学院精细化工研究所(王钒，陈仰三，董黎雾)，上海医科大学(庄庆祺) |
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| 摘 要： | 在室温下,采用聚丙烯酰胺凝胶电泳法,分离经荧光胺或对氯荧光胺荧光标记的蛋白质样品,可在2.5小时内完成蛋白质样品的分离和其区带的鉴定。耗时是传统方法的四分之一,而且荧光图象清晰、灵敏度较高。
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| 关 键 词： | 特效试剂 蛋白 聚丙烯酰胺 凝胶电泳 荧光标记 分析 荧光胺 对氯荧光胺 |
Fluorometric assay of fluorescence-labeled proteins in the polyacrylamide gel electrophoresis systems |
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| Wang Fan,Chen Yangsan,Dong Lifen,Zhuang Qingqi East China Institute of Chemical Technology,Shanghai Shanghai Medical University. Fluorometric assay of fluorescence-labeled proteins in the polyacrylamide gel electrophoresis systems[J]. Journal of East China University of Science and Technology, 1987, 0(5) |
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| Authors: | Wang Fan Chen Yangsan Dong Lifen Zhuang Qingqi East China Institute of Chemical Technology Shanghai Shanghai Medical University |
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| Abstract: | The isolation of fluorescamine or p-chlorofluorescamine-labeled proteins in the po-lyacrylamide gel electrophoresis systems can be carried out at room temperature. A pro-tein sample can be isolated and its discs identified within two hours and a half, time thusspent being only a quarter as much as that of the traditional method and identification ofsharp and well-defined protein discs made possible with a much higher sensitivity. |
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| Keywords: | fluorometric protein polyacrylamide gel electrophoresis fluorescence labelling analysis fluorescamine p-chlorofluorescamine |
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