活性污泥制氢系统中发酵细菌的分离与鉴定

分离鉴定高效产氢发酵细菌是发酵发生物制氢技术的重要前提,利用Hungater技术与宽体窄颈培养瓶平板技术,以及LM-1和HPB-LR培养基分离鉴定厌氧发酵产氢细菌获得六株产氢细菌,葡萄糖是他们最适宜的底物.他们的产氢代谢为乙醇发酵型.产氢细菌发酵液相末端产物分析表明乙醇和乙酸占其总代谢产物的95?%.生理生化和形态学特征分析表明它们属于一种特殊的微生物类群.16SrDNA碱基序列分析表明它们可以划分为新的种属.

Isolation and characterization of several fermentative H2-producing bateria in hydrogen production system by actvitaed sludge

The isolation and identification of high efficient biohydrogen production anaerobic bacteria are important foundation of fermented biohydrogen production process by anaerobic digesting organic waste water. The 6 strains of H2 - producing bacteria were isolated by media (LM-1 and HPB - LR) . Improved Hungater rolling tube technique and plate method of culture bottle (PM-CB) were employed to enumerate and isolate the HPB. Glucose is optimum cobon source and showed ethanol fermentation type in H2 - producing metabolic pathway. The end liquid products were at protion of 95%-99% . Physiological , biochemical and morphorlogical traits showed that the bacteria belong to special group. Identity analysis of 16s rDNA sequence indicated that the group maybe a new genus.