| Differentiation of embryonic stem cells transfected by ibeB gene |
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| 引用本文： | SHANGDeshu FANGWengang CHENYuhua. Differentiation of embryonic stem cells transfected by ibeB gene[J]. 科学通报(英文版), 2005, 50(5): 421-424. DOI: 10.1007/BF02897456 |
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| 作者姓名： | SHANGDeshu FANGWengang CHENYuhua |
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| 作者单位： | DepartmentofDevelopmentalBiology,KeyLabofCellBiology,MinistryofPublicHealthofChina,ChinaMedicalUniversity,Shenyang110001,China |
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| 摘 要： | We have previously identified an E. coli determinant, ibeB gene locus contributing to invasion of human brain microvascular endothelial cells. In the present study, we established embryonic stem (ES) cell lines overexpressing IbeB and found that exogenic ibeB gene could start-up expression of a neural stem cell specific marker, nestin, and give rise to polar changes. In analysis of IbeB location, it was found that GFP-IbeB fusion protein targeted at the ES cell nucleus. These data suggests that ibeB gene may play an important role in the regulation of nestin expression.
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| 关 键 词： | 胚胎细胞 ibeB基因 神经细胞 嵌套模式 交互作用 病原菌 蛋白质 真核细胞 |
| 收稿时间： | 2004-07-14 |
Differentiation of embryonic stem cells transfected byibeB gene |
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| Deshu Shang,Wengang Fang,Yuhua Chen. Differentiation of embryonic stem cells transfected byibeB gene[J]. Chinese science bulletin, 2005, 50(5): 421-424. DOI: 10.1007/BF02897456 |
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| Authors: | Deshu Shang Wengang Fang Yuhua Chen |
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| Affiliation: | (1) Department of Developmental Biology, Key Lab of Cell Biology, Ministry of Public Health of China, China Medical University, 110001 Shenyang, China |
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| Abstract: | We have previously identified an E. coli determinant, ibeB gene locus contributing to invasion of human brain microvascular endothelial cells. In the present study, we established embryonic stem (ES) cell lines overexpressing IbeB and found that exogenic ibeB gene could start-up expression of a neural stem cell specific marker, nestin, and give rise to polar changes. In analysis of IbeB location, it was found that GFP-IbeB fusion protein targeted at the ES cell nucleus. These data suggests that ibeB gene may play an important role in the regulation of nestin expression. |
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| Keywords: | embryonic stem cell ibeB gene cell differentiation neural cell nestin. |
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