| 荚膜红细菌接合转移系统的建立及优化 |
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| 引用本文： | 吴海珍,李莉,张惠展,刘和.荚膜红细菌接合转移系统的建立及优化[J].华东理工大学学报(自然科学版),2000,26(1):45-47. |
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| 作者姓名： | 吴海珍 李莉 张惠展 刘和 |
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| 作者单位： | 1. 华东理工大学生物工程学院,上海,200237
2. 苏州第四制药厂,江苏,215008 |
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| 基金项目： | 中国科学院资助项目,29776017, |
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| 摘 要： | 从培养方式及培养时间对光合细菌Ｒｈｏｄｏｂａｃｔｅｒ ｃａｐｓｕｌａｔｕｓ的ＤＮＡ接合转移系统进行了优化,研究了该接合转移系统的部分特性,发现接合质粒的大小、受体菌细胞膜的特性对接合效率影响较明显,这为光合细菌基因工程菌的构建提供了基础。
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| 关 键 词： | 接合转移 接合效率 培养 优化 荚膜红细菌 |
| 修稿时间： | 1998-03-10 |
Establishment and Optimization of DNA Conjugation of Rhodobacter capsulatus |
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| WU Hai zhen ,LI Li ,ZHANG Hui zhan ,LIU He.Establishment and Optimization of DNA Conjugation of Rhodobacter capsulatus[J].Journal of East China University of Science and Technology,2000,26(1):45-47. |
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| Authors: | WU Hai zhen LI Li ZHANG Hui zhan LIU He |
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| Institution: | WU Hai zhen 1*,LI Li 1,ZHANG Hui zhan 1,LIU He 2 |
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| Abstract: | DNA conjugation of R.capsulatus B10 was established. The conjugation efficiency was optimized by culture type and culture time. Some factors such as the size of plasmids affected the efficiency strongly were found. These provide the basis of construction of genetic strains. |
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| Keywords: | Rhodobacter capsulatus conjugation conjugation efficiency cuture type and time optimised |
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