| 3-溴-5-氯水杨醛缩邻氯苯胺与DNA作用的紫外光谱研究 |
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| 引用本文： | 蒲小华. 3-溴-5-氯水杨醛缩邻氯苯胺与DNA作用的紫外光谱研究[J]. 宝鸡文理学院学报(自然科学版), 2012, 32(2): 31-33,51 |
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| 作者姓名： | 蒲小华 |
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| 作者单位： | 宝鸡文理学院化学化工系,陕西宝鸡,721013 |
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| 基金项目： | 宝鸡文理学院重点项目(ZK11147) |
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| 摘 要： | 目的研究3-溴-5-氯水杨醛缩邻氯苯胺(简写为BCCA)与小牛胸腺DNA(CT-DNA)的作用机制。方法利用紫外光谱、黏度法确定BCCA与CT-DNA的相互作用方式。结果 BCCA的紫外吸收光谱随CT-DNA浓度的增加表现出明显的增色效应和较小的红移;BCCA存在时,CT-DNA的熔点升高;CT-DNA的黏度随着BCCA浓度的增大而增大。结论 BCCA以插入方式与DNA相互作用。
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| 关 键 词： | 3-溴-5-氯水杨醛缩邻氯苯胺 小牛胸腺DNA 插入结合 |
Study on the interaction between 3-bromo-5-chlorosalicylaldehyde-o-chloroaniline and DNA by ultraviolet spectroscopy |
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| PU Xiao-hua. Study on the interaction between 3-bromo-5-chlorosalicylaldehyde-o-chloroaniline and DNA by ultraviolet spectroscopy[J]. Journal of Baoji College of Arts and Science(Natural Science Edition), 2012, 32(2): 31-33,51 |
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| Authors: | PU Xiao-hua |
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| Affiliation: | PU Xiao-hua(Dept.Chem.& Chem.Eng.,Baoji Univ.Arts & Sci.,Baoji 721013,Shaanxi,China) |
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| Abstract: | Aim To study the action mechanism of 3-bromo-5-chlorosalicylaldehyde-o-chloroaniline(BCCA) with calf thymus DNA(CT-DNA).Methods Ultraviolet spectroscopy and viscosity measurement were used to study the interaction mode of BCCA with CT-DNA.Results Ultraviolet absorption spectrum of BCCA produced significant hyperchromic effect and slight bathochromic shift with the increase of the concentration of CT-DNA;the melting point was higher when BCCA existed;the CT-DNA viscosity increased with the increase of the concentration of BCCA.Conclusion BCCA interacts with CT-DNA in the mode of intercalation. |
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| Keywords: | 3-bromo-5-chlorosalicylaldehyde-o-chloroaniline CT-DNA intercalation |
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