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| 高效液相色谱法测定鲑鱼降钙素及其注射液中含量 | |
| 引用本文： | 王玉,LI Ying-sing,张正行,安登魁,赵祥大.高效液相色谱法测定鲑鱼降钙素及其注射液中含量[J].安徽大学学报(自然科学版),2000,24(4):85-91. |
| 作者姓名： | 王玉 LI Ying-sing 张正行 安登魁 赵祥大 |
| 作者单位： | 1. Department of Chemistry of The University of Memphis, TN 38152, U.S. A 2. 中国药科大学药分教研室，江苏南京 210009 3. 安徽大学化学系，安徽合肥 230039 |
| 摘 要： | 选择优化的HPLC色谱条件 ,用梯度洗脱法分离测定原药及注射液中鲑鱼降钙素的含量。以Vydac 2 1 8TP54C1 8柱 (4.6× 2 50mm ,30 0 0A ,5μm )作分析柱 ,流动相∶A液为 0 .0 5mol L磷酸二氢钠缓冲液 (pH =3.0 ) ,B液为A液 +乙腈 (2∶3)。梯度洗脱∶B液在 2 0min内从 45%增加至 70 %。检测波长为 2 1 5nm。方法的精密度、线性关系、回收率均良好 ,和生物测定法相比较 ,本法简便、快速、经济 ,测定结果较为满意。并用建立的方法考察原药及其注射液的稳定性。 |
| 关 键 词： | 高效液相色谱法 梯度洗脱法 鲑鱼降钙素 注射液 稳定性 |
| 文章编号： | 1000-2162(2000)04-0085-07 |
Determination of Calcitonin and Its Content in Injection by HPLC |
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| WANG Yu,LI Ying-sing,ZHANG Zhen-xin,AN mng-qui,ZHAO Xiang-da.Determination of Calcitonin and Its Content in Injection by HPLC[J].Journal of Anhui University(Natural Sciences),2000,24(4):85-91. | |
| Authors: | WANG Yu LI Ying-sing ZHANG Zhen-xin AN mng-qui ZHAO Xiang-da |
| Abstract: | A gradient HPLC method was developed for the determination of calcitonin and its content in injection. A Vydac 218T54 C18 column was used and the followed solutions was used as mobile phase: A is 0.05mol/L NaH2PO4 (pH3.0), B is A+ acetonitrile (2:3). B solution was changed to 70% from 45% between 0～20 minutes. The UV detector wavelength was 215nm. The precision, linear relationship and recovery were good respectively. Compared with bioassay, the pro posed method was faster, more convenient and economical as well as with satisfactory results. The stabilities of Calcitonin and its injection were studied by this method. |
| Keywords: | HPLC gradient elution calcitonin calcitonin injection stability |
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