Localization of peroxidase activity inTrypanosoma cruzi microbodies |
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| Authors: | R. Docampo J. F. de Boiso A. Boveris A. O. M. Stoppani |
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| Affiliation: | (1) Facultad de Medicina, Instituto de Quimica Biologica, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, (Argentina) |
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| Abstract: | Summary Electron microscopic observation ofTrypanosoma cruzi epimastigotes reveals the presence of microbody-like structures (microperoxisomes) in which 3,3 -diaminobenzidine (DAB) is peroxidized to electron-opaque material. The role of peroxidase in DAB peroxidation is supported by the enzyme demonstration in disrupted epimastigotes and the microbody-containing cell fractions.This investigation was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and the Special Fund for the Study of Chagas Disease (Subsecretaría de Ciencia y Tecnología de la Nación). |
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