| 壳聚糖的溶菌酶降解 |
| |
| 引用本文： | 马如,黄明智.壳聚糖的溶菌酶降解[J].北京化工大学学报(自然科学版),2002,29(6):40-43. |
| |
| 作者姓名： | 马如 黄明智 |
| |
| 作者单位： | 北京化工大学材料科学与工程学院,北京,100029 |
| |
| 摘 要： | 用粘度法和还原糖法研究了溶菌酶降解壳聚糖过程中温度、pH值、时间、酶浓度、底物浓度以及金属离子对降解速度的影响.比较合适的降解条件是:温度45℃,pH值5.0,适当增大酶浓度和底物浓度能够加速壳聚糖的降解,在一定的底物浓度下溶菌酶降解壳聚糖的反应不遵循简单的一级反应动力学.一定浓度的Cu2+、Zn2+、Ba2+、Cr3+能够促进壳聚糖在溶菌酶作用下的降解,而K+、Ca2+和高浓度的Zn2+会抑制酶活力的发挥.
|
| 关 键 词： | 壳聚糖 溶菌酶 降解 |
| 修稿时间： | 2002年3月29日 |
Hydrolysis of chitosan with lysozyme |
| |
| MA Ru,HUANG Ming-zhi.Hydrolysis of chitosan with lysozyme[J].Journal of Beijing University of Chemical Technology,2002,29(6):40-43. |
| |
| Authors: | MA Ru HUANG Ming-zhi |
| |
| Institution: | College of Materials Science and Engineering; Beijing University of Chemical Technology; Beijing; China |
| |
| Abstract: | The effect s of reaction temperature , pH , time , subst rate and enzyme concent rations , as well as metalions on the hydrolytic rate of chitosan by lysozyme were investigated. The optimum temperature and optimumpH were 45 ℃and 5. 0 respectively. Increasing the concent rations of subst rate and enzyme solutions could accelerate the hydrolysis of chitosan. The depolymerization of chitosan didn’t conform to the simple enzymic kinetics model within certain subst rate concent rations. A certain concent ration range of Cu2+ , Zn2+ , Ba2+ or Cr3+ cangreatly improve the enzymic activity of lysozyme , while K+ , Ca2 + and a high concent ration of Zn2+ will inhibit the enzymic activity. |
| |
| Keywords: | chitosan lysozyme hydrolysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《北京化工大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《北京化工大学学报(自然科学版)》下载免费的PDF全文 |
