人胃腺癌细胞系恶性表型逆转过程中细胞骨架变化的初步研究

应用显示细胞骨架的光镜和电镜方法观察表明,MGc80-3细胞质内微管很少,中间纤维数量稀少、构型改变,质膜内缘有较丰富的微丝层,具有恶性细胞典型的、不发达的细胞骨架特征.但经dBcAMP诱导后,细胞质内微管和中间纤维数量增多,分布排列有规则,质膜内缘微丝大量减少,细胞骨架组成、构型、数量与分布均产生与正常细胞大体相似的恢复性改变.这种变化是由于dBeAMP诱导胃癌细胞内cAMP水平的提高而实现的.细胞骨架正常构型和功能的恢复,对于逆转细胞形态结构的改变、细胞增殖的调控和细胞表面特性的改变均具有重要影响,是癌变细胞恶性表型逆转的一种重要的形态和功能表现.

Preliminary Studies on the Cytoskeleton Changes During Malignant Phenotypic Reversion of Human Gastric Adenocarcinoma Cell Line MGc80-3

Light and electron microscopic studies of cytoskeleton indicated that. in MGc80 3 cells, there were a few microtubules at cytoplasm and abundant microfilaments within cytomembrane, the intermediate filaments were rare and their configuration varied. They had a typical and nondeveloPmental cytoskeleton feature of malignant cells. But after inducing treatment with dBcAMP, the microtubules and intermediate filaments at cytoplasm increased. distributed and arranged regularly, and the microfilaments within cytomembrane reduced in a large degree. The composition, configuration quaptity and distribution of their cytoskeleton all emerged a restorative alternation similar to those of normal cells. These changes were realized by the enhancing of cAMP level in gastric carcionma cells induced by dBcAMP. The restoration of normal configuration and function of cytoskeleton had an important influence on the changes of cellular shape and structure as well as the regulation and controlling of cellular multiplication and the characteristic alternation of cell surface in the reversed cells. It is an important morphological and functional expression to the malignant PhenotyPic reversion of cancer-ational cells.