| 金铁锁总皂苷含量测定 |
| |
| 引用本文： | 王垄,周欣. 金铁锁总皂苷含量测定[J]. 贵州师范大学学报(自然科学版), 2012, 30(3): 4-6 |
| |
| 作者姓名： | 王垄 周欣 |
| |
| 作者单位： | 1. 贵州大学精细化工研究开发中心,贵州贵阳550025／贵州师范大学天然药物质量控制研究中心,贵州贵阳550001
2. 贵州师范大学天然药物质量控制研究中心,贵州贵阳550001／贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001 |
| |
| 基金项目： | 贵州省科技创新人才团队建设项目,贵州省中药现代化科技产业研究开发专项项目,贵阳市科学技术计划项目,贵阳市科技计划项目 |
| |
| 摘 要： | 建立金铁锁总皂苷的含量测定方法。方法:以齐墩果酸为对照品,5%香草醛冰醋酸、高氯酸为显色剂,采用分光光度法,在540nm处测定吸光度,计算金铁锁中总皂苷的含量。结果:金铁锁总质量浓度在1～10μg.mL-1与吸光度呈良好的线性关系(r=0.998 4),平均回收率为97.9%(n=6),RSD=1.61%;金铁锁中总皂苷含量四川最高,云南次之,贵州最低。结论:建立了金铁锁中总皂苷超声提取含量测定方法,此方法简便、准确,稳定性好、可用于金铁锁药材的质量控制。
|
| 关 键 词： | 金铁锁 总皂苷 分光光度法 |
Determination of total saponins in Psammosilene tunicoides |
| |
| WANG Long,ZHOU Xin. Determination of total saponins in Psammosilene tunicoides[J]. Journal of Guizhou Normal University(Natural Sciences), 2012, 30(3): 4-6 |
| |
| Authors: | WANG Long ZHOU Xin |
| |
| Affiliation: | 2,3*(1.Fine Chemicals Research and Development Center,Guizhou University,Guiyang,Guizhou 550025,China; 2.Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University,Guiyang,Guizhou 550001,China;3.School of Chemistry and Material Science,Guizhou Normal University,Guiyang,Guizhou 550001,China) |
| |
| Abstract: | To establish the determination methods of total saponins from Psammosilene tunicoides.Methods: The oleanolic acid was selected as comparison product and 5% vanillin-acetic acid-HCLO solution was selected as color-producing reagent.Determiningn absorbability in the point of 540nm by spectrophotometry method.And calculating the total saponins in Psammosilene tunicoides.Results: The calibration curve was linear over the range of 1~10μg.mL-1 with the correlation of 0.998 4,the average recoveries of dioscin was 97.9%(n=6),RSD=1.61%;the highest content of total saponins is Sichuan,Yunnan,followed by Guizhou minimum.Conclusion: Psammosilene tunicoides of ultrasonic extraction of total saponin assay method is simple,accurate,good stability.It can be used for quality control of medicinal Psammosilene tunicoides. |
| |
| Keywords: | Psammosilene tunicoides total saponin spectrophotometric method |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
