| Polarized synchronous light scattering characterization of the interaction of proteins with sodium dodecyl sulfonate |
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| 作者姓名： | ZHAO XiaoHui HUANG ChengZhi |
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| 作者单位： | College of Chemistry and Chemical Engineenng, Southwest University, Chongqing 400715, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China （Grant Nos. 30570465 and 20425517） |
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| 摘 要： | In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobulin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were con- sistent with the data measured by dynamic light scattering (DLS) technique.
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| 关 键 词： | 人血清白蛋白 免疫球蛋白 蛋白质 十二烷基磺酸钠 相互作用 偏振光散射 |
| 收稿时间： | 15 June 2006 |
| 修稿时间： | 2006-06-152006-11-07 |
Polarized synchronous light scattering characterization of the interaction of proteins with sodium dodecyl sulfonate |
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| ZHAO XiaoHui HUANG ChengZhi.Polarized synchronous light scattering characterization of the interaction of proteins with sodium dodecyl sulfonate[J].Chinese Science Bulletin,2007,52(4):456-460. |
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| Authors: | Zhao XiaoHui Huang ChengZhi |
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| Institution: | (1) College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China |
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| Abstract: | In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobulin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were con- sistent with the data measured by dynamic light scattering (DLS) technique. |
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| Keywords: | human serum albumin immunoglobulin G sodium dodecyl sulfonate polarization light scattering |
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