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| Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly（ethylene glycol） and polyethyleneimin | |
| 作者姓名： | ZHOU JianHua HUANG Lu WANG WeiWei PANG Jun ZOU Yang SHUAI XinTao GAO Xin |
| 作者单位： | [1]Department of Urology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China [2]BME Center, Schoolof Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China [3]Department of Radiology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China |
| 基金项目： | Supported by the National Natural Science Foundation of China (Grant Nos. 30772178, 50673103), Natural Science Foundation of Guangdong Province (Grant Nos. 7117362, 7003703) and Key Clinical Program of Ministry of Health Founda-tion of China |
| 摘 要： | Methoxyl poly （ethylene glycol） （mPEG-OH） was successfully grafted onto branched polyethyleneimine （hy-PEI） to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package superparamagnetic iron oxide （SPIO） by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex （mPEI-g-PEG-SPIO） surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen （PSCA）. Our study showed that the single chain antibody functionalized nanoprobe （scAbpscA-PEI-g-PEG-SPIO） with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%, Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer （PCa）. |
| 关 键 词： | 超顺磁性氧化铁 甲氧基聚乙二醇 接枝共聚物 纳米探针 前列腺癌 MRI 磁性纳米颗粒 单克隆抗体 |
| 收稿时间： | 2008-11-24 |
| 修稿时间： | 2009-03-02 |
Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly(ethylene glycol) and polyethyleneimin |
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| ZHOU JianHua,HUANG Lu,WANG WeiWei,PANG Jun,ZOU Yang,SHUAI XinTao,GAO Xin.Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly(ethylene glycol) and polyethyleneimin[J].Chinese Science Bulletin,2009,54(18):3137-3146. | |
| Authors: | JianHua Zhou Lü Huang WeiWei Wang Jun Pang Yang Zou XinTao Shuai Xin Gao |
| Institution: | 1 Department of Urology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; 2 BME Center, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China; 3 Department of Radiology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China |
| Abstract: | Methoxyl poly (ethylene glycol) (mPEG-OH) was successfully grafted onto branched polyethyleneimine (hy-PEI) to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package super- paramagnetic iron oxide (SPIO) by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex (mPEI-g-PEG-SPIO) surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen (PSCA). Our study showed that the single chain antibody functionalized nanoprobe (scAbPSCA-PEI-g-PEG-SPIO) with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%. Our results revealed that the potential of this magnetic nanopar- ticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer (PCa). |
| Keywords: | PEG-grafted-hyperbranched-PEI (PEG-g-PEI) prostate cancer (PCa) prostate stem cell antigen (PSCA) superparamagnetic iron oxide nanoparticles (SPIO) magnetic resonance imaging (MRI) |
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