Cloning of a novel gene associated with human nasopharyngeal carcinoma |
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Authors: | Zhiwei He Lu Xie Liangguo Xu Ke Lan Weidong Liu Ling Zhang Caiping Ren Jianling Shi Wen Zhou Kaitai Yao |
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Affiliation: | (1) Key Laboratory of Carcinogenesis of Chinese Ministry of Health, Cancer Research Institute of Hunan Medical University, 410078 Changsha, China |
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Abstract: | One EST N27741 with high expression in normal adult nasopharynx tissues but low expression in adult poorly differentiated squamous nasopharyngeal carcinoma has been selected out by the high-density cDNA array expression profiling technique. The differential expression has been confirmed by RT-PCR. One novel gene of 1096 bp has been cloned based on this EST. Bioinformatics analysis found that the new gene sequence contains a whole reading frame encoding 256 amino acids. There is a stop codon TAA in front of the 5′ end start codon, and a tailing signal AATAAA and poly A tail at the 3′ end. There is no homologous known gene found after searching by blasting this sequence to non-redundancy nucleotide database. Therefore it is considered a novel gene related to nasopharyngeal carcinoma. |
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Keywords: | nasopharyngeal carcinoma novel gene cloning |
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