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中国沿海真鲷群体遗传多样性的同工酶电泳分析
引用本文:王志勇,王艺磊,林利民,张子平,洪惠馨,张雅芝,于涛,张俭. 中国沿海真鲷群体遗传多样性的同工酶电泳分析[J]. 集美大学学报(自然科学版), 2003, 8(3): 213-217
作者姓名:王志勇  王艺磊  林利民  张子平  洪惠馨  张雅芝  于涛  张俭
作者单位:集美大学水产学院、水产生物技术研究所,福建,厦门,361021
基金项目:农业部重点科技项目(渔95-A-96-01-02),福建省科技项目资助(98-Z-106)
摘    要:用同工酶电泳技术研究威海、厦门、北部湾3个海区真鲷群体的遗传多态性和群体之间的遗传趋异,共观测了LDH、MDH和EST3种酶11个基因位点,其中LDH的3个位点在3个群体都为单态,MDH有1个位点、EST有2—4个位点表现多态,3个群体多态位点数量和平均杂合度以威海群体最高(45.5%与0.161),厦门群体次之(27.3%与0.156),北部湾群体最低(27.3%与0.120),北部湾与厦门海区的真鲷在遗传上较为相似,遗传距离0.012,威海海区真鲷与二者的差异较大,遗传距离分别达0.021(与厦门标本)和0.025(与北部湾标本)。

关 键 词:中国沿海 真鲷 群体遗传多态性 同工酶电泳 基因位点 遗传趋异 遗传距离
文章编号:1007-7405(2003)03-0213-05
修稿时间:2003-04-18

Studies on the Genetic Polymorphisms of Red Sea Bream Stocks from the Coastal Waters of China Using Isoenzyme Analysis
WANG Zhi-yong,WANG Yi-lei,LIN Li-min,ZHANG Zi-ping,HONG Hui-xin,Zhang Ya-zhi,YU Tao,ZHANG Jian. Studies on the Genetic Polymorphisms of Red Sea Bream Stocks from the Coastal Waters of China Using Isoenzyme Analysis[J]. the Editorial Board of Jimei University(Natural Science), 2003, 8(3): 213-217
Authors:WANG Zhi-yong  WANG Yi-lei  LIN Li-min  ZHANG Zi-ping  HONG Hui-xin  Zhang Ya-zhi  YU Tao  ZHANG Jian
Abstract:Genotypic and allelic frequencies were examined at 11 enzymatic loci in three stocks of red sea bream ( Pagrosomus major) from the coastal waters of China by using vertical polyacrylamide gel electro-phoresis. Polymorphic loci (major allele frequency less than 0. 95 ) were found in MDH and EST, and 3-5 loci were polymorphic in each stock, i. e, m-Mdh, Est-6, Est-4, Est-3 and Est-2. The proportion of polymorphic loci and average heterozygosity of the 3 stocks were 27. 3 % and 0. 120 in Beibuwan stock, 27. 3 % and 0. 156 in Xiamen stock, 45. 5 % and 0. 161 in Weihai one, respectively. Comparison of allele frequencies coupled with estimates of the genetic distance indicated that the Weihai stock is significantly different from the others, and the genetic diversity between Beibuwan and Xiamen stock is not significant.
Keywords:red sea bream  isoenzyme  electrophoresis  genetic polymorphism
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