| 口服感染昆虫纯化重组杆状病毒 |
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| 引用本文： | 施先宗 庞义. 口服感染昆虫纯化重组杆状病毒[J]. 华中师范大学学报(自然科学版), 1996, 30(2): 207-211 |
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| 作者姓名： | 施先宗 庞义 |
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| 作者单位： | 华中师范大学昆虫学研究所，中山大学昆虫学研究所生物防治国家重点实验室 |
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| 摘 要： |
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| 关 键 词： | 口服感染 昆虫幼虫 杆状病毒 纯化效率 重组病毒 |
PURIFICATION OF RECOMBINANT BACULOVIRUS WITH POLYHEDRON BY PER OS INFECTION OF INSECT LARVAE |
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| Shi Xianzong,Chen Quhou. PURIFICATION OF RECOMBINANT BACULOVIRUS WITH POLYHEDRON BY PER OS INFECTION OF INSECT LARVAE[J]. Journal of Central China Normal University(Natural Sciences), 1996, 30(2): 207-211 |
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| Authors: | Shi Xianzong Chen Quhou |
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| Abstract: | Constuction of transfer plasmids that help recombinant baculoviruses produce polyhedra facilitates the purification of recombinant baculoviruses. Insect larvae were per os infected in this experiment with the resultant of the cotransfection of viral DNA and Plasmid DNA, and the hemolymph of diseased larvae were collected. Plaque assay showed that about 90% prgeny viruses produced in the larvae were the expected recombinant baculovirus. Another turn of plaque purification gave 100% recombinant baculoviruses. It decreases the cost and the time by this way compared to the conventional plaque assay procedure. |
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| Keywords: | per os infection insect larvae recombinant baculovirus purification efficiency |
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