| 脱水胰凝乳蛋白酶制备方法的改进 |
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| 引用本文： | 刘莹,程洪强,张姝,周慧,李惟. 脱水胰凝乳蛋白酶制备方法的改进[J]. 吉林大学学报(理学版), 1999, 0(1): 4 |
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| 作者姓名： | 刘莹 程洪强 张姝 周慧 李惟 |
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| 作者单位： | 吉林大学生命科学学院,长春,130023 |
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| 摘 要： | 对脱水胰凝乳蛋白酶（ＡｎＣｈｔ）的制备方法进行改进．在粗品ＡｎＣｈｔ亲和层析纯化前再进行一次ＰＭＳＦ化学修饰，降低ＡｎＣｈｔ中残存的蛋白水解酶活力．分析表明，用该方法制得的ＡｎＣｈｔ与抑制剂ＢＰＴＩ结合的化学计量及强度均接近于理论值，并且其催化活力几乎全部丧失，表明纯度很高，可用于Ｃｈｔ与其底物间的分子识别
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| 关 键 词： | 脱水胰凝乳蛋白酶 胰凝乳蛋白酶 苯甲基磺酰氟(PMSF) 化学修饰 |
| 修稿时间： | 1998-03-16 |
An Improved Method for the Preparation of Anhydrochymotrypsin |
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| Liu Ying,Cheng Hongqiang,Zhang Shu,Zhou Hui,Li Wei. An Improved Method for the Preparation of Anhydrochymotrypsin[J]. Journal of Jilin University: Sci Ed, 1999, 0(1): 4 |
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| Authors: | Liu Ying Cheng Hongqiang Zhang Shu Zhou Hui Li Wei |
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| Abstract: | We improved the usual method for the preparation of anhydrochymotrypsin (AnCht). Before the crude AnCht was purified by affinity chromatography, it was chemically modified by PMSF once again. This strategy greatly reduced the residual proteinase activity of the product. The further analysis demonstrated that the product can bind to chymotrypsin inhibitor BPTI stoichiometrically and the purity of it is high. Therefore, it can be used in the studies of molecular recognition between chymotrypsin and its substrates. |
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| Keywords: | anhydrochymotrypsin chymotrypsin PMSF chemical modification |
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