华美游仆虫皮层细胞骨架的超微结构及免疫电镜观察

 应用透射和免疫电镜术，在超微结构水平观察显示，腹毛类纤毛虫华美游仆虫的皮层纤毛器和非纤毛器细胞骨架中，表膜下、细胞质深部的微管或纤维束，以及纤毛器及纤毛器附属微管等均是以α-和 β-微管蛋白为主要成分的微管类结构；中心蛋白定位于纤毛器的基体腔、基体基部近中心端、基体基部近中心端连接及基体中部连接结构中，而在纤毛杆和皮层非纤毛器微管中无中心蛋白成分存在。据此认为，该纤毛虫中，基体部位的蛋白组成机制与纤毛杆及纤毛器附属结构是不同的，非分裂期细胞中定位于基体主体部位的中心蛋白可能与维持相关结构的稳定性有关。

Ultrastructure and Immunoelectron Microscopic Observations on Cortical Microtubular Cytoskeleton of Euplotes elegans(Ciliophora: Hypotrichida)

Cortical microtubular cytoskeleton and immunocytolocalization of α-and β-tubulin and centrin were revealed by using transmission electron microscopy and immunoelectron microscopy. The results show that the cortical ciliature and cortical non-ciliated regions cytoskeleton of Euplotes elegans are composed of subpellicular and deep cytoplasmic microtubules or fiber bundles, ciliature and ciliature base-associated microtubules. These microtubules and fiber bundles are mainly constructed by α-and β-tubulin; centrin is localized in the basal body lumen, proximal level of the basal bidies(PLBB), links located at the PLBB and fibrous links located at the median level of basal bodies of ciliature, but no decoration at all has been detected within the ciliary shafts and cortical non-ciliated regions microtubules. It is concluded that the organizing mechanisms are different between basal body and ciliary shafts or ciliature base-associated microtubules of Euplotes elegans. Centrin which localized at basal body of interphase cell may play a key role in the stability maintenance of basal body microtubules and links of basal body.