五味子中残留农药对卵巢颗粒细胞的毒性研究

探讨五味子中残留农药对卵巢颗粒细胞的毒性作用.MTT法测定农药提取物作用于卵巢颗粒细胞24、48、72 h后的细胞增殖情况;Western blot法检测农药提取物对卵巢颗粒细胞MAPK信号通路的ERK1/2及p-ERK1/2蛋白表达水平的影响.MTT结果显示农药提取物能够显著促进卵巢颗粒细胞增殖,48 h增殖率最高(P0.01),Western blot结果显示提取物能够促进ERK1/2、p-ERK1/2蛋白表达,与阴性对照组相比差异极显著(P0.01).因此农药提取物作用于体外培养的卵巢颗粒细胞能够显著促进细胞增殖,通过促进MAPK信号通路的ERK1/2、p-ERK1/2蛋白表达,对颗粒细胞产生毒性作用.

Research on toxic effect of pesticide residues from schisandra on ovarian granulose cells

The research method of the pesticide residues toxic effect from schisandra on the o-varian granulose cell was explored .MTT assay was used to determine pesticide extract on o-varian granulosa cells after 24, 48,72 h cell proliferation,and Western blot was used to de-tect the impact of pesticide extract on the expression levels of ERK 1/2 and p-ERK1/2 pro-tein of MAPK signal pathway of ovarian granulosa cell .MTT results showed that pesticide ex-tract could significantly promote cell proliferation , and the highest growth rate was at 48 h （ P 〈0.01 ） .Western blot showed that pesticide extract could promote the expression of ERK1/2 and p-ERK1/2 protein , which compared with the negative control group had sig-nificant difference （ P 〈0 .01 ） .Therefore pesticides extract on ovarian granulosa cells in vitro could significantly promote cell proliferation by promoting the expression of ERK 1/2 , p-ERK1/2 protein of MAPK signal pathway to produce toxic effects for granulosa cells .