| A novel gene carrier based on amino-modified silica nanoparticles |
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| 作者姓名： | HEXiaoxiao WANGKemin |
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| 作者单位： | HE Xiaoxiao，WANG Kemin，TAN Weihong，LIU Bin，LIN Xia，HUANG Shasheng，LI Du，HE Chunmei & LI Jun State Key Laboratory of Chemo/Biosensing and Chemometrics，College of Chemistry & Chemical Engineering，Institute of Biological Technol-ogy，Hunan University，Changsha 410082，China |
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| 基金项目： | 科技部"攀登计划"，国家自然科学基金，国家杰出青年科学基金，教育部重点项目，教育部高校骨干教师资助计划，国家海外青年学者联合基金，湖南省自然科学基金 |
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| 摘 要： | Uniform-sized amino-modified silica nanoparticles have been prepared by the controlled synchronous hydrolysis of tetraethoxysilane and N-(β-amimoethyl)-γ-aminopropyltriethoxysilane in water nanodroplet of the water-in-oil microemulsion.These nanoparticles display positive charge potential at definited pH,This is due to the presence of amino groups on the surface of the nanoparticles,Nanoparticles-plasmid DNA complexes can easily form through electrostatical binding between the positive charges of the amino-modified silica nanoparticles and the negative charges of the plasmid DNA,The complexes can be also dissociated under alkaline pH or high ionic strength conditions.And enzymatic digestion of the plasmid DNA is almost inhibited by these nanoparticles complexes.A novel non-viral gene carrier based on the amino-modified silica nanoparticles is proposed under the combination of nanotechnology,biotechnology and gene engineering technology.The plasmid DNA can successfully cross various systemic barriers to COS-7 cells as well as mediate high expression of Green Fluorescence Protein(GFP)gene in cells by use of this novel gene carrier.
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| 关 键 词： | 非病毒基因载体 氨基修饰硅氧颗粒 纳米粒子 转基因技术 质粒DNA |
| 收稿时间： | 2002-07-02 |
A novel gene carrier based on amino-modified silica nanoparticles |
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| He,Xiaoxiao,Wang,Kemin,Tan,Weihong,Liu,Bin,Lin,Xia,Huang,Shasheng,Li,Du,He,Chunmei,Li,Jun.A novel gene carrier based on amino-modified silica nanoparticles[J].Chinese Science Bulletin,2003,48(3):223-228. |
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| Authors: | He Xiaoxiao Wang Kemin Tan Weihong Liu Bin Lin Xia Huang Shasheng Li Du He Chunmei Li Jun |
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| Institution: | HE Xiaoxiao, WANG Kemin, TAN Weihong, LIU Bin, LIN Xia, HUANG Shasheng, LI Du, HE Chunmei & LI Jun State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technol-ogy, Hunan University, Changsha 410082, China |
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| Abstract: | Science Bulletin - Uniform-sized amino-modified silica nanoparticles have been prepared by the controlled synchronous hydrolysis of tetraethoxysilane and... |
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| Keywords: | gene carrier nanoparticle amino-modified silica |
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