| Inhibiting effect of melittin on pathogens of crops |
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| 引用本文： | PAN LingZi NA Jie XING Zhuo FANG HongJun WANG GuanLin. Inhibiting effect of melittin on pathogens of crops[J]. 科学通报(英文版), 2007, 52(5): 639-644. DOI: 10.1007/s11434-007-0117-0 |
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| 作者姓名： | PAN LingZi NA Jie XING Zhuo FANG HongJun WANG GuanLin |
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| 作者单位： | College of Life Science, Liaoning Normal University, Dalian 116029, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China （Grant No. 30170112）, and the International Cooperation Project （Grant No. 30310103130） |
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| 摘 要： | The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.
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| 关 键 词： | 蜂毒肽 作物 病原菌 抑制作用 抑菌机制 抗菌谱 |
| 收稿时间： | 2006-09-01 |
| 修稿时间： | 2006-09-012007-01-05 |
Inhibiting effect of melittin on pathogens of crops |
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| Pan LingZi,Na Jie,Xing Zhuo,Fang HongJun,Wang GuanLin. Inhibiting effect of melittin on pathogens of crops[J]. Chinese science bulletin, 2007, 52(5): 639-644. DOI: 10.1007/s11434-007-0117-0 |
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| Authors: | Pan LingZi Na Jie Xing Zhuo Fang HongJun Wang GuanLin |
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| Affiliation: | (1) College of Life Science, Liaoning Normal University, Dalian, 116029, China |
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| Abstract: | The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency. |
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| Keywords: | melittin crop pathogens factors bacteriostatic mechanism |
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