| Inhibiting effect of melittin on pathogens of crops |
| |
| 作者姓名： | PAN LingZi NA Jie XING Zhuo FANG HongJun WANG GuanLin |
| |
| 作者单位： | College of Life Science, Liaoning Normal University, Dalian 116029, China |
| |
| 基金项目： | Supported by the National Natural Science Foundation of China （Grant No. 30170112）, and the International Cooperation Project （Grant No. 30310103130） |
| |
| 摘 要： | The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.
|
| 关 键 词： | 蜂毒肽 作物 病原菌 抑制作用 抑菌机制 抗菌谱 |
| 收稿时间： | 1 September 2006 |
| 修稿时间： | 2006-09-012007-01-05 |
Inhibiting effect of melittin on pathogens of crops |
| |
| PAN LingZi NA Jie XING Zhuo FANG HongJun WANG GuanLin.Inhibiting effect of melittin on pathogens of crops[J].Chinese Science Bulletin,2007,52(5):639-644. |
| |
| Authors: | Pan LingZi Na Jie Xing Zhuo Fang HongJun Wang GuanLin |
| |
| Institution: | (1) College of Life Science, Liaoning Normal University, Dalian, 116029, China |
| |
| Abstract: | The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency. |
| |
| Keywords: | melittin crop pathogens factors bacteriostatic mechanism |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
