| 拟南芥Rad23原核重组蛋白构建和活性检测 |
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| 引用本文： | 李秀山,刘斌,赵李剑,唐东英,赵小英,刘选明.拟南芥Rad23原核重组蛋白构建和活性检测[J].湖南大学学报(自然科学版),2011,38(11):66-69. |
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| 作者姓名： | 李秀山 刘斌 赵李剑 唐东英 赵小英 刘选明 |
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| 作者单位： | 湖南大学生物学院,湖南长沙,410082 |
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| 基金项目： | 国家自然科学基金资助项目(30770200) |
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| 摘 要： | 在拟南芥中,Rad23基因通过参与泛素/26s蛋白酶体途径调节植物生长发育和激素应答.为进一步研究该基因编码蛋白的分子作用机制及其生物学功能,构建了pCold-Rad23融合蛋白重组表达载体,将该载体转化到大肠杆菌BL21中诱导表达目的蛋白.SDS聚丙烯酰胺凝胶电泳检测结果表明,分子质量为94 ku左右的融合重组蛋白在...
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| 关 键 词： | 蛋白 泛素/26S蛋白酶体 原核表达 抗体 |
Purification of Arabidopsis Rad23 Recombinant Protein in Engineering Bacteria and Preparation of Its Polyclonal Antibody |
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| LI Xiu-shan,LIU Bin,ZHAO Li-jian,TANG Dong-ying,ZHAO Xiao-ying,LIU Xuan-ming.Purification of Arabidopsis Rad23 Recombinant Protein in Engineering Bacteria and Preparation of Its Polyclonal Antibody[J].Journal of Hunan University(Naturnal Science),2011,38(11):66-69. |
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| Authors: | LI Xiu-shan LIU Bin ZHAO Li-jian TANG Dong-ying ZHAO Xiao-ying LIU Xuan-ming |
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| Abstract: | Rad23 gene which participates in ubiquitin/26S proteasome pathway in Arabidopsis,is known to play important roles in plant development and responses to hormone.In order to study its function and molecular mechanism,an expression vector pCold-Rad23 was constructed and transformed into E.coli BL21.Then the recombinant fusion protein was induced by IPTG.The results of SDS-PAGE have shown that Rad23 fusion protein existed in a soluble form with a relative molecular mass 94 ku,which is fit for the molecular mass... |
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| Keywords: | protein ubiquitin(Ub)/26S proteasome prokaryotic expression antibodies |
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