| 紫芝液体深层发酵条件的初步研究 |
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| 引用本文： | 付丽红,王金菊,郝利民,王艳萍.紫芝液体深层发酵条件的初步研究[J].天津科技大学学报,2013(5):19-22,27. |
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| 作者姓名： | 付丽红 王金菊 郝利民 王艳萍 |
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| 作者单位： | 1. 食品营养与安全教育部重点实验室，天津科技大学食品工程与生物技术学院，天津 300457
2. 中国人民解放军总后勤部军需装备研究所,北京,100010 |
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| 基金项目： | 全军科研重点项目“军用功能基础原料生物制备技术研究” |
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| 摘 要： | 以紫芝为研究对象,讨论液体深层发酵碳源、氮源、无机盐和培养条件对菌体生物量的影响,确定紫芝液体发酵最优培养基配方为葡萄糖30,g/L、蛋白胨3,g/L、KH2PO41.2,g/L和MgSO4·7H2O 0.8,g/L.发酵条件为培养温度25,℃,起始pH自然,装液量24%,接种量15%,140,r/min培养7,d.同时,以菌体生物量、胞外多糖和pH为指标,绘制紫芝液态发酵动力学曲线,发酵培养7,d后菌体生物量和胞外粗多糖的含量分别达到最大值3.63,g/L和1.67,g/L.
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| 关 键 词： | 紫芝 深层发酵 菌体生物量 胞外多糖 |
Preliminary Study of Liquid Submerged Fermentation of Ganoderma sinensis |
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| FU Lihong
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WANG Jinju
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HAO Limin
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WANG Yanping.Preliminary Study of Liquid Submerged Fermentation of Ganoderma sinensis[J].Journal of Tianjin University of Science & Technology,2013(5):19-22,27. |
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| Authors: | FU Lihong WANG Jinju HAO Limin WANG Yanping |
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| Institution: | 1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457, China; 2.The Quartermaster Institute of the General Logistics Department ofP.L.A, Beijing 100010, China) |
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| Abstract: | The effect of nutritional and environmental conditions on mycelial biomass of Ganoderma sinensis in liquid fer- mentation were studied with a single factor and an orthogonal experiment. The results show that the best carbon source and nitrogen source are glucose and peptone respectively. Consequently, the optimal culture medium components were：30 g/L glucose, 3 g/L peptone, 1.2 g/L KH2PO4and 0.8 g/L MgSO4·7H2O. The optimum culture conditions were as follows：the liquid medium volume was 24%, inoculation quantity 15%, culture temperature 25 ℃, shaking frequency 140 r/min, and pH value was natural The liquid fermentation kinetics curve was determined by biomass, crude extrapolysaccharide and pH, and the optimization fermentation time was 7 d. Then the mycelium yield and extrapolysaccharide could reach 3.63 g/L and 1.67 g/L, respectively. |
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| Keywords: | Ganoderma sinensis submerged fermentation mycelial biomass extrapolysaccharide |
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