| Impact of nuclear actin on the prophase chromosome construction of Physarum polycephalum |
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| 作者姓名： | JING Yuqi WANG Xiaoguang HAO Shui ZENG Xianlu |
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| 作者单位： | Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China,Department of Biology, Changchun Normal College, Changchun 130032, China,Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China,Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金 |
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| 摘 要： | A cell-free system efficiently promoting mitosis has been developed using the precise natural synchronous plasmodium of Physarum polycephalum. The content changes of nuclear cyclin B were exploited to represent the prophase process of Physarum polycephalum. The possible function of nuclear actin on chromosome construction was investigated by detecting the content changes of nuclear cyclin B in the late G2 phase nuclei treated with cytochalasin B and incubated in the cell-free system. Our results showed that nuclear actin plays an important role in the process of the chromosome construction.
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| 关 键 词： | Physarum polycephalum nuclear actin chronosome construction cyclin B cell-free system |
Impact of nuclear actin on the prophase chromosome construction of Physarum polycephalum |
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| JING Yuqi,WANG Xiaoguang,HAO Shui,ZENG Xianlu.Impact of nuclear actin on the prophase chromosome construction of Physarum polycephalum[J].Progress in Natural Science,2004,14(9):781-785. |
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| Authors: | JING Yuqi WANG Xiaoguang HAO Shui ZENG Xianlu |
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| Institution: | 1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
2. Department of Biology, Changchun Normal College, Changchun 130032, China |
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| Abstract: | A cell-free system efficiently promoting mitosis has been developed using the precise natural synchronous plasmodium of Physarum polycephalum. The content changes of nuclear cyclin B were exploited to represent the prophase process of Physarum polycephalum. The possible function of nuclear actin on chromosome construction was investigated by detecting the content changes of nuclear cyclin B in the late G2 phase nuclei treated with cytochalasin B and incubated in the cell-free system. Our results showed that nuclear actin plays an important role in the process of the chromosome construction. |
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| Keywords: | Physarum polycephalum nuclear actin chronosome construction cyclin B cell-free system |
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