| 脂肪酶干燥方法对环十五内酯合成的影响 |
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| 引用本文： | 刘雄民,郭辰,沈芳,梁静娟,马丽.脂肪酶干燥方法对环十五内酯合成的影响[J].广西大学学报(自然科学版),2007,32(2):130-133. |
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| 作者姓名： | 刘雄民 郭辰 沈芳 梁静娟 马丽 |
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| 作者单位： | 广西大学化学化工学院,广西南宁,530004 |
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| 基金项目： | 教育部科学技术研究重点项目
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广西自然科学基金 |
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| 摘 要： | 研究了冷冻干燥法,真空干燥法,N2吹干法3种干燥方法对Candida.sp.GXU08所产脂肪酶活力的影响,以3种方法制得的酶为催化剂进行了15-羟基十五烷酸甲酯环化反应.结果表明,冷冻干燥的酶粉酶活保留率最高,有78.74%,转化率为1.367%;真空干燥酶活保留率有75%,转化率为1.158%;N2吹干法的酶活保留率最低为63.9%,转化率仅有0.405%.
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| 关 键 词： | 脂肪酶 环十五内酯 15-羟基十五烷酸甲酯 干燥 |
| 文章编号： | 1001-7445（2007）02-0130-04 |
| 修稿时间： | 2007-04-212007-04-27 |
The effect of lipase drying methods on the synthesis of cyclopentadecanolide |
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| LIU Xiong-min,GUO Chen,SHEN Fang,LIANG Jing-juan,MA Li.The effect of lipase drying methods on the synthesis of cyclopentadecanolide[J].Journal of Guangxi University(Natural Science Edition),2007,32(2):130-133. |
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| Authors: | LIU Xiong-min GUO Chen SHEN Fang LIANG Jing-juan MA Li |
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| Institution: | College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004, China |
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| Abstract: | The effect of the freezing drying methods,the vacuum-drying,the N2 blowing law on activity of lipase to Candida.sp.GXU08 was studied,methyl 15-Hydroxy-pentadecanate was cyclized by lipase as catalyst.The result showed that the retention rate of activity of lipase of enzyme powder was the highest with the freeze-drying,was 78.74%,freezing drying also had the highest conversion,was 1.367%;the retention rate of activity of lipase of enzyme powder was 75% with vacuum drying,conversion was 1.158%;the N2 blowing law was the lowest in the retention rate of activity of lipase,was 63.9%,conversion only came to 0.405%. |
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| Keywords: | lipase cyclopentadecanolide methyl 15-Hydroxy-pentadecanate drying |
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