| 中温α-淀粉酶基因的克隆及在枯草芽孢杆菌中的高效表达 |
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| 引用本文： | 刘逸寒,路福平,王建玲,胡博.中温α-淀粉酶基因的克隆及在枯草芽孢杆菌中的高效表达[J].天津科技大学学报,2011(4):1-5. |
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| 作者姓名： | 刘逸寒 路福平 王建玲 胡博 |
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| 作者单位： | 工业发酵微生物教育部重点实验室天津科技大学生物工程学院; |
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| 基金项目： | 天津市科技攻关重点培育项目(06YFGPSH03500); 天津科技大学自然科学基金资助项目(20090205) |
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| 摘 要： | 利用高效表达栽体pWB980,实现了Bacillus subtilis BF7658中温α-淀粉酶基因amy在B.subtilis DB403中的高效表达,活力达到770 U/mL.经多步纯化,重组酶AMY的比活达到35.8 U/mg,纯化倍数为1.7,获得凝胶电泳条带单一的蛋白样品,经SDS-PAGE检测,重组酶AM...
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| 关 键 词： | 枯草芽孢杆菌 中温α-淀粉酶 高效表达 纯化 酶学性质 |
High-Level Expression of the Medium-Temperature Alpha Amylase in Bacillus subtilis |
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| LIU Yi-han,LU Fu-ping,WANG Jian-ling,HU Bo.High-Level Expression of the Medium-Temperature Alpha Amylase in Bacillus subtilis[J].Journal of Tianjin University of Science & Technology,2011(4):1-5. |
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| Authors: | LIU Yi-han LU Fu-ping WANG Jian-ling HU Bo |
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| Institution: | LIU Yi-han,LU Fu-ping,WANG Jian-ling,HU Bo(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | The medium-temperature alpha amylase gene from Bacillus subtilis BF768 was cloned into vector pWB980 and high-level expressed in B.subtilis DB403.The activity of AMY in the supernatant of the culture medium reached a maximum of 770 U/mL.By multi-step purification,the specific activity of AMY was up to 35.8 U/mg with a 1.7-fold purification.The recombinant enzyme AMY had a molecular mass of 4.8×104.The optimum pH and temperature of AMY was 6.0 and 60 ℃. |
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| Keywords: | Bacillus subtilis medium-temperature alpha amylase high-level expression purification enzyme properties |
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