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| 原癌基因c-jun对EDS诱导大鼠睾丸间质细胞凋亡的研究 | |
| 引用本文： | 徐斯凡,袁双虎,王晶磊.原癌基因c-jun对EDS诱导大鼠睾丸间质细胞凋亡的研究[J].中央民族大学学报(自然科学版),2006,15(3):197-202. |
| 作者姓名： | 徐斯凡 袁双虎 王晶磊 |
| 作者单位： | 1. 中央民族大学,生命与环境科学学院,北京,100081 2. 山东省肿瘤防治研究院,山东,济南,250117 3. 南昌大学医学院,江西,南昌,330063 |
| 摘 要： | 本研究用c-junASODNs拮抗c-jun,用EDS诱导的大鼠睾丸间质细胞凋亡,通过琼脂糖凝胶电泳、Henchest33342染色荧光显微镜检查方法研究c-jun对EDS诱导的大鼠睾丸间质细胞凋亡的影响.实验结果显示0.5μmol/Lc-junASODNs抑制EDS诱导的离体间质细胞凋亡(P<0.01).提示c-jun有促进大鼠睾丸间质细胞凋亡的作用. |
| 关 键 词： | 睾丸间质细胞 反义寡脱氧核苷酸 凋亡 |
| 文章编号： | 1005-8036（2006）03-0197-06 |
| 收稿时间： | 2006-03-15 |
| 修稿时间： | 2006年3月15日 |
Effect of c-jun on Apoptosis of Rat Leydig Cells in vitro |
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| XU Si-fan,YUAN Shuang-hu,WANG Jing-lei.Effect of c-jun on Apoptosis of Rat Leydig Cells in vitro[J].Journal of The Central University for Nationalities(Natural Sciences Edition),2006,15(3):197-202. | |
| Authors: | XU Si-fan YUAN Shuang-hu WANG Jing-lei |
| Institution: | 1. College of Life and Environmental sciences, Central University for Nationalities, Beijing 100081, China ; 2. Shandong Tumor Hospital Institute, Jinan ,250117, China ; 3. Medical college of Nanchang University, Nanchang 330006, China |
| Abstract: | Apoptosis is necessary for the occurance and development of Leydig cells.The effects of c-jun on apoptpsis of rat Leydig cells(LC) by antisense oligodeoxynucleotides(ASODNs) in vitro were studied in this paper.The apoptosis was identified in isolated rat Leydig cells by using Henchest33342 nucleus staining and DNA fragmentation agarose gel electrophoresis.The results showed that c-jun ASODNs decreased EDS-induced apoptosis of LC.It is suggested that c-jun proto-oncogene could enhance the apoptosis of Leydig cells. |
| Keywords: | c-jun |
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