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向日葵耐盐突变体筛选
引用本文:刘海学,李雪松,苏明,刘鹏,刘海臣.向日葵耐盐突变体筛选[J].内蒙古民族大学学报(自然科学版),2008,23(2):161-164.
作者姓名:刘海学  李雪松  苏明  刘鹏  刘海臣
作者单位:1. 内蒙古民族大学,农学院,内蒙古,通辽,028042;天津农学院,天津,300384
2. 天津优联投资发展集团有限公司,天津,300141
3. 大连大学,辽宁,大连,116622
4. 内蒙古民族大学,农学院,内蒙古,通辽,028042
摘    要:以MS为基本培养基,研究了不同激素对康地5号向日葵愈伤组织的诱导效果,筛选出了最佳诱导愈伤组织培养基;以子叶、下胚轴及真叶作为外植体。确定了子叶和下胚轴作为康地5号组织培养的最佳外植体;在含50、100、及150mmol/L NaCl不同盐浓度培养基上分别诱导出向日葵耐盐愈伤组织,继代培养后得到不同耐盐程度的新株系,移栽后得到完整植株.

关 键 词:向日葵  耐盐突变体  愈伤组织  筛选
文章编号:1671-0185(2008)02-0161-03
修稿时间:2007年12月20

Screening of Sunflower Mutant with Salt-tolerant Ability
LIU Hai-xue,LI Xue-song,SU Ming,LIU Pen,LIU Hai-chen.Screening of Sunflower Mutant with Salt-tolerant Ability[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2008,23(2):161-164.
Authors:LIU Hai-xue  LI Xue-song  SU Ming  LIU Pen  LIU Hai-chen
Institution:LIU Hal - xue, LI Xue - song, SU Ming, LIU Pen, LIU Hal - chen (1 Agricultural College, Inner Mongolia University for Nationalities, Tongliao 028042, China; 2. Tianjin Agricultural University, Tianjin 300384 ,China; 3. Tianjin Youlian Invest Development Croup Limited Company, Tianjin 300141 ,China; 4. Dalian University, Dalian 116622,China)
Abstract:The effects of different plant hormones on the sunflower callus induction were investigated with MS as the basic culture medium. Consequently , the best culture medium for callus induction was selected out . Compared with Cotyledons , euphyllas, and Hypocotyls , Cotyledons and Hypocotyls was the best explant for sunflower tissue culture. The callus of sunflower which was induced from the medium containing 50, 100, or 150mmol/L NaCl was successfully regenerated and transferred into the soil .
Keywords:Sunflower  Mutant with Salt - tolerant  Callus  Screening
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