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| CdS纳米晶标记抗雌二醇抗体荧光免疫分析试剂 | |
| 引用本文： | 王传涛,王术皓,杜凌云,庄惠生.CdS纳米晶标记抗雌二醇抗体荧光免疫分析试剂[J].河北师范大学学报(自然科学版),2007,31(2):218-221. |
| 作者姓名： | 王传涛 王术皓 杜凌云 庄惠生 |
| 作者单位： | 1. 聊城大学,化学化工学院,山东,聊城,252059 2. 聊城大学,化学化工学院,山东,聊城,252059;东华大学,环境科学与工程学院,上海,200051 3. 东华大学,环境科学与工程学院,上海,200051 |
| 基金项目： | 国家自然科学基金 , 山东省自然科学基金 |
| 摘 要： | 在水相中制备了CdS纳米晶,其在536 nm处有较强荧光发射,用巯基乙酸修饰后与抗雌二醇抗体偶联,制备了CdS纳米晶标记的抗雌二醇抗体标记物,该标记物具有良好的荧光特性,发射波长红移至588 nm.利用该标记物竞争免疫分析法测定雌二醇的线性范围为0.20～8.00 mg/L. |
| 关 键 词： | CdS纳米晶 荧光 抗雌二醇抗体 免疫分析试剂 |
| 文章编号： | 1000-5854(2007)02-0218-04 |
| 修稿时间： | 2006-07-10 |
Preparation of the Fluorescence Immunoassay Reagent of Estradiol by Using CdS Semiconductor Nanocrystals as Label |
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| WANG Chuan-tao,WANG Shu-hao,DU Ling-yun,ZHUANG Hui-sheng.Preparation of the Fluorescence Immunoassay Reagent of Estradiol by Using CdS Semiconductor Nanocrystals as Label[J].Journal of Hebei Normal University,2007,31(2):218-221. | |
| Authors: | WANG Chuan-tao WANG Shu-hao DU Ling-yun ZHUANG Hui-sheng |
| Institution: | 1. College of Chemistry and Chemical Engineering, Liaocheng University, Shandong Liaocheng 252059, China; 2. College of Environmental Science and Engineering, Donghua University, Shanghai 200051, China |
| Abstract: | The CdS semiconductor nanoerystals was synthesis in water phase, and it has strong fluorescence emission at 536 nm after modified by mereaptoaeetie acid. The binding of the anti-estradiol antibody with CdS nanocrystals was prepared, and this binding had fluorescence characteristics. An competitive immunoassay method was developed for detecting estradiol by using the synthesized reagent, the linear range for determination of estradiol is 0.20-8.00 mg/L. The result showed that it could be used for determination of estradiol as fluorescence immunoassay reagent. |
| Keywords: | CdS semiconductor nanocrystals fluorescence immunoassay labeling estradiol |
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