Sr2+ can become incorporated into an agonist-sensitive,cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium |
| |
Authors: | W. H. Ko J. D. Pediani D. L. Bovell S. M. Wilson |
| |
Affiliation: | (1) Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, West Medical Building, G12 8QQ Glasgow, (Scotland, UK);(2) Department of Biological Sciences, Glasgow Caledonian University, G13 1PP Glasgow, (Scotland, UK);(3) Department of Physiology, The Chinese University of Hong Kong, (Hong Kong) |
| |
Abstract: | We have explored the properties of a Ca2+-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca2+-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+. |
| |
Keywords: | Ca2+ mobilisation purinergic receptors nucleotide receptors sweat gland epithelial cells ionomycin Fura-2 horse |
本文献已被 SpringerLink 等数据库收录! |
|