| 1株桃果实采后病原真菌的鉴定以及碳源代谢指纹图谱分析 |
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| 引用本文： | 王友升,郭晓敏,姚子鹏,李丽萍. 1株桃果实采后病原真菌的鉴定以及碳源代谢指纹图谱分析[J]. 北京工商大学学报(自然科学版), 2011, 29(1): 47-53 |
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| 作者姓名： | 王友升 郭晓敏 姚子鹏 李丽萍 |
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| 作者单位： | 北京工商大学食品学院/食品添加剂与配料北京高校工程研究中心,北京,100048 |
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| 摘 要： | 从采后贮藏过程中发病的八月脆桃果实(Amygdalus persica cv.Bayuecui)中分离到1株真菌菌株074#.通过对病原菌形态学观察以及核糖体ITS rDNA序列分析,证明菌株074#为灰葡萄孢霉菌(Botrytis elliptica).致病性试验结果表明,桃果实为灰葡萄孢霉菌的寄主.通过Biolog FFMicroPlate分析该病原菌对95种碳源的利用能力,结果表明,Botrytis elliptica代谢指纹图谱为最适碳源包括D-果糖、蔗糖、L-苹果酸、琥珀酸、D-葡萄糖酸、L-天门冬氨酸、L-丝氨酸、腺苷、5'-磷酸腺苷等26种,可利用碳源14种,不可利用碳源55种.
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| 关 键 词： | 桃果实 病原真菌 ITSrDNA序列分析 碳源代谢指纹图谱 |
Identification and Carbon Metabolic Fingerprinting Analysis of a Pathogen Isolated from Postharvest Peach Fruit |
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| WANG You-sheng,GUO Xiao-min,YAO Zi-peng and LI Li-ping. Identification and Carbon Metabolic Fingerprinting Analysis of a Pathogen Isolated from Postharvest Peach Fruit[J]. Journal of Beijing Technology and Business University:Natural Science Edition, 2011, 29(1): 47-53 |
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| Authors: | WANG You-sheng GUO Xiao-min YAO Zi-peng LI Li-ping |
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| Affiliation: | WANG You-sheng,GUO Xiao-min,YAO Zi-peng,LI Li-ping (School of Food/Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Technology and Business University,Beijing 100048,China) |
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| Abstract: | A fungal pathogen,strain 074#,was isolated from infected peach fruit during postharvest storage periods.Morphological characterization and ITS rDNA analysis confirmed that the strain 074# was Botrytis elliptica.The pathogenicity tests confirmed that Amygdalus persica is a natural host of Botrytis elliptica.The Biolog Microbial Identification System with FF MicroPlate was applied for carbon source utilization test of Botrytis elliptica based on their abilities to utilize 95 discrete substrates.And the result... |
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| Keywords: | peach fruit fungal pathogen ITS rDNA sequence analysis carbon metabolic fingerprinting |
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