| 两种玉米DNA提取方法的比较 |
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| 引用本文： | 李桂玲,李欢庆. 两种玉米DNA提取方法的比较[J]. 河南科学, 2005, 23(5): 686-688 |
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| 作者姓名： | 李桂玲 李欢庆 |
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| 作者单位： | 河南工业大学生物工程学院,河南,郑州,450052;河南工业大学生物工程学院,河南,郑州,450052 |
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| 摘 要： | 本试验用改良的CTAB法和煮沸法两种方法提取玉米基因组DNA.紫外光分光光度法和0.8%水平琼脂糖凝胶电泳检测结果表明:改良的CTAB法虽然步骤多,但要比煮沸法所提的DNA的质量和纯度都要高,且都符合一般分子生物学反应DNA的最佳质量要求.
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| 关 键 词： | 玉米 DNA 提取 |
| 文章编号： | 1004-3918(2005)05-0686-03 |
| 收稿时间： | 2005-04-30 |
| 修稿时间： | 2005-04-30 |
The comparison of both extraction methods of maize DNA |
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| LI Gui-ling,LI Huan-qing. The comparison of both extraction methods of maize DNA[J]. Henan Science, 2005, 23(5): 686-688 |
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| Authors: | LI Gui-ling LI Huan-qing |
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| Abstract: | In this experiment, both the modified CTAB method and the boiling method were used to extract the maize total genome DNA. The results showed that the steps of improved CTAB method was much more complex, but the concentration and purity of DNA was higher than that of the boiling, which matched with optimal molecular biological request. |
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| Keywords: | maize DNA extraction method |
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