非洲菊组培快繁技术

以非洲菊幼嫩花托为外植体,采用MS作为基本培养基,添加不同生长激素对其进行组织培养研究。结果表明：非洲菊幼小花托MS＋BA3.0mg/L＋NAA0.2mg/L培养基上能很好地诱导形成愈伤组织并分化出不定芽,诱导率50%;继代培养基为：MS＋BA0.5mg/L＋NAA0.2mg/L,增殖系数2.8;生根培基为1/2MS＋NAA0.5mg/L,生根率可达90%。

Research on Tissue Culture of Gerbera Jamesonii Bolus

Taking the young receptacle of Gerbera Jamesonii Bolus as the explants, this paper studies the tissue culture by adopting MS as the basic culture medium and adding different growth hormones. The results show that MS BA 3.0 mg/L NAA 0.2mg/L of small receptacle of Gerbera Jamesonii Bolus can commendably induce callus for producing the shoots through young thalamus explants, and the rate of inducement was 50%; in gerbera proliferation media ,the effect of combining with BA 0.5 mg/L NAA 0.2 mg/L was better than others, and on an average,2.8 shoots were formed per original explants; the root media was 1/2MS NAA0.5 mg/L, the rate of root was 90%.