Enzyme immunoassay for arginine vasopressin |
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| Authors: | T Uno K Uehara K Motomatsu E Ishikawa K Kato |
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| Institution: | (1) Department of Anesthesiology and Biochemistry, Medical College of Miyazaki, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, 889-16 Miyazaki, (Japan);(2) Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, 480-03 Kasugai, Aichi, (Japan) |
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| Abstract: | Summary A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was  -D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube. |
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