Plant aquaporin selectivity: where transport assays, computer simulations and physiology meet |
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Authors: | Uwe Ludewig Marek Dynowski |
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Institution: | 1. Applied Plant Sciences, Institute of Botany, Darmstadt University of Technology, Schnittspahnstr. 10, 64287, Darmstadt, Germany 2. Center for Plant Molecular Biology, Plant Physiology, Auf der Morgenstelle 1, 72076, Tübingen, Germany
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Abstract: | Plants contain a large number of aquaporins with different selectivity. These channels generally conduct water, but some additionally
conduct NH3, CO2 and/or H2O2. The experimental evidence and molecular basis for the transport of a given solute, the validation with molecular dynamics
simulations and the physiological impact of the selectivity are reviewed here. The aromatic/arginine (ar/R) constriction is
most important for solute selection, but the exact pore requirements for efficient conduction of small solutes remain difficult
to predict. Yeast growth assays are valuable for screening substrate selectivity and are explicitly shown for hydrogen peroxide
and methylamine, a transport analog of ammonia. Independent assays need to address the relevance of different substrates for
each channel in its physiological context. This is emphasized by the fact that several plant NIP channels, which conduct several
solutes, are specifically involved in the transport of metalloids, such as silicic acid, arsenite, or boric acid in planta. |
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