| 青菜花粉管通道法导入外源DNA的研究初报 |
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| 引用本文： | 王光清 李美珍. 青菜花粉管通道法导入外源DNA的研究初报[J]. 复旦学报(自然科学版), 1996, 35(2): 127-131 |
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| 作者姓名： | 王光清 李美珍 |
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| 作者单位： | 复旦大学遗传学研究所，闸北区彭浦乡 |
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| 基金项目： | 上海市科学技术发展基金 |
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| 摘 要： | 采用花粉管通道法，将青菜株系７５Ｆ５、１４号大白菜、紫阳等三个品种的总ＤＮＡ及质粒ｐＡｃｔ１－Ｄ ＤＮＡ导入青菜６０５品系，收获青菜种子。种子发芽后分别提取约两周苗龄的小苗总ＤＮＡ，并经ＥｃｏＲⅠ酶切，以ｇｕｓ基因片段为探针做子杂交，证实了外源ｇｕｓ基因已整合到青菜６０５品系中；田间观察还获得了一株与供体大白菜花形相似的变异株。
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| 关 键 词： | 青菜 分子育种 花粉管通道 外源DNA导入 育种 |
Preliminary study of the introduction of foreign DNAs into cabbage(Brassica chinensis L.) via pollen-tube pathway |
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| Wang Guangqing, Wang Yunzhu, Hu Rongxia, Yang Jinshui, Ge Koulin. Preliminary study of the introduction of foreign DNAs into cabbage(Brassica chinensis L.) via pollen-tube pathway[J]. Journal of Fudan University(Natural Science), 1996, 35(2): 127-131 |
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| Authors: | Wang Guangqing Wang Yunzhu Hu Rongxia Yang Jinshui Ge Koulin |
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| Abstract: | otal DNAs of Brassica chinensis L. strain 76F5, Brassica pekinensis L.,Brassica oleracea var rubra L. and plasmid pActl-D were introduced into Brassica chinensis L. variety 605 via pollen-tube pathway. Seeds from the foreign DNA-treated plants were harvested and allowed to germinate. Hybridization of the gus gene fragment probe to products of EcoR I-digested total DNAs from various plants were carried out and hybridization bands were detected in the total DNAs of progenies from foreign DNA-treated plants. A variant similar to the donor Brassica pekinensis L. was obtained among progenies. |
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| Keywords: | cabbage molecular breeding pollen-tube pathway introduction of foreign DNA variant plant. |
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