| 樱桃番茄的组织培养与离体快繁研究 |
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| 引用本文： | 丁兰,梁桂霞,杨永利. 樱桃番茄的组织培养与离体快繁研究[J]. 西北师范大学学报(自然科学版), 2002, 38(2): 64-67 |
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| 作者姓名： | 丁兰 梁桂霞 杨永利 |
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| 作者单位： | 西北师范大学生命科学学院,西北师范大学生命科学学院,西北师范大学生命科学学院 甘肃兰州 730070,甘肃兰州 730070,甘肃兰州 730070 |
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| 摘 要： | 以樱桃番茄（L.Esculutum v.Cerasiforme）的下胚轴、幼叶、茎尖及带芽茎段为外植体,在附加不同浓度BA和LAA的MS培养基上进行离体培养,结果表明：樱桃番茄“红宝石”品种的下胚轴、叶和茎尖的最佳芽诱导培养基分别为MS 2.0mg/L BA 0.5mg/LIAA;MS 2.0mg/L BA 0.2mg/LIAA;MS 1.0～2.0mg/L BA 0.1～0.5mg/LIAA。芽最佳增殖培养基MS 1.0mg/L BA 0.5mg/LIAA,其增殖系数为3～4,用带侧芽的无菌短枝进行离体扦插,可1次成苗,扦插培养基为MS 0.005～0.010mg/LNAA或不加激素,其增殖系数可达5。
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| 关 键 词： | 离体快繁 樱桃番茄 组织培养 无性繁殖系 培养基 离体培养 离体扦插 |
| 文章编号： | 1001-988X(2002)02-0064-04 |
| 修稿时间： | 2001-11-26 |
The study of tissue culture and rapid propagation of cherry tomato |
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| DING Lan,LIANG Cui-xia,YANG Yong-li. The study of tissue culture and rapid propagation of cherry tomato[J]. Journal of Northwest Normal University Natural Science (Bimonthly), 2002, 38(2): 64-67 |
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| Authors: | DING Lan LIANG Cui-xia YANG Yong-li |
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| Abstract: | Four kinds of tissue of Lycopersicon Esculentum var. Cerasiforme, hypocotyl, leaf, shoot-tip, and stem with a lateral bud cut from cultivars "Hong Bao-shi" are cultured on MS medium with BA and JAA at different levels. The results show that the optimal induction media of buds are MS + 1 .0- 2.0 mg/L BA + 0.5 mg/L IAA, MS + 2.0 mg/L BA+0.2 mg/L LAA, MS + 1.0.- 2.0 mg/L BA + 0.1 -.0.5 mg/LIAA. Proliferation medium of shoots is MS + 1.0 mg/L BA + 0.5 mg/L IAA. Induction of mot is better for shoots in MS + 0. 1 mg/L NAA. The cuttage of the stem in vitro are carried on MS + 0.005 -0.01 mg/L NAA or MS without hormone. The rate of proliferation of the shoots is 5. |
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| Keywords: | Lycopersicon Esculuntum var. Cerasiforme tissue culture clonal shoot-tip leaf |
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