超临界CO2萃取-高速逆流色谱分离纯化欧前胡素和异欧前胡素

研究了用超临界CO₂萃取法从白芷中提取欧前胡素和异欧前胡素，根据正交试验设计确定了超临界萃取最佳工艺条件为温度60 ℃、萃取压力25 MPa和药材粉碎度40~60目，并放大50倍进行萃取试验。利用高速逆流色谱技术分离纯化了欧前胡素和异欧前胡素，溶剂系统为正己烷:乙酸乙酯:甲醇:水(1:0.4:1.1:0.5，V/V)。采用HPLC-DAD，MS对欧前胡素和异欧前胡素进行了定性定量分析，得到产物纯度达99.0%以上，本方法简便快捷，重现性好。

Separation and purification of imperatorin and isoimperatorin with supercritical CO2 fluid extraction- high-speed counter-current chromatography

This paper addressed the extraction of imperatorin and isoimperatorin from Angelica dahurica （Fisch. ex Hoffm. ） with supercritJcal CO2 extraction method. Orthogonal experiments show that the optimal technological conditions of this extraction method are temperature of 60 ℃, pressure of 2.5 MPa, sample particle size of 40 -60 mesh and 50 times amplification of a preparative supercritical fluid extraction （SFE） system. We separated and purified imperatorin and isoimperatorin with high-speed counter-current chromatography and a two-phase solvent system of 1 ： 0.4： 1. 1： 0.5 （ n- hexane-ethyl vs. acetate vs. methanol vs. water, V/V）. We also employed HPLC-DAD and MS to perform quantative and qualitive analysis for imperatorin and isoimperatorin. The purities of them were both above 99.0%. The method is convenient and fast,with a high reproducibility.