| 对虾白斑综合症病毒P9蛋白转录水平的微阵列分析及其免疫荧光标记 |
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| 引用本文： | 吴常嵩,谢希贤,杨丰.对虾白斑综合症病毒P9蛋白转录水平的微阵列分析及其免疫荧光标记[J].厦门大学学报(自然科学版),2006,45(2):268-271. |
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| 作者姓名： | 吴常嵩 谢希贤 杨丰 |
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| 作者单位： | 1. 厦门大学生命科学学院,福建,厦门,361005
2. 国家海洋局第三海洋研究所,福建,厦门,361005 |
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| 基金项目： | 国家新技术研究发展基金(863计划)，中国科学院资助项目 |
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| 摘 要： | 从构建的对虾白斑综合症病毒cDNA文库中,我们筛选到一个拷贝数最高,编码82个氨基酸的基因(命名为p9,wsv230).该基因被克隆到pGEX-2T载体中进行GST融合蛋白的原核表达,纯化的融合蛋白GST-P9用于制备特异的多克隆抗体.利用微阵列技术和免疫荧光标记技术,对该蛋白的转录水平及其在感染细胞内的分布进行了初步研究,表明该基因为高丰度表达,推测是对虾白斑综合症病毒的一个重要基因.
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| 关 键 词： | 对虾白斑综合症病毒 p9基因 微阵列 免疫荧光标记 |
| 文章编号： | 0438-0479(2006)02-0268-04 |
| 收稿时间： | 04 6 2005 12:00AM |
| 修稿时间： | 2005年4月6日 |
Microarray-based Gene Expression Analysis of p9 Protein of White Spot Syndrome Virus (WSSV) and Its Immunofluorescent Labeling |
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| WU Chang-song,XIE Xi-xian,YANG Feng.Microarray-based Gene Expression Analysis of p9 Protein of White Spot Syndrome Virus (WSSV) and Its Immunofluorescent Labeling[J].Journal of Xiamen University(Natural Science),2006,45(2):268-271. |
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| Authors: | WU Chang-song XIE Xi-xian YANG Feng |
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| Abstract: | White spot syndrome virus(WSSV) has been the major pathogen that led to severe mortalities of the cultured shrimps in recent years.An open reading frame(termed the p9 gene) encoding 82 amino acids was revealed,which was most abundant in the WSSV cDNA library.The gene was cloned into pGEX-2T and expressed as a fusion protein with glutathione S-transterase(GST).Specific antibody was raised by using the purified fusion protein(GST-p9).With the technique of microarray and immunofluorescent labeling,the gene expression level of p9 protein and its location in the infected cells were studied.The results showed that p9 gene was highly expressed and may be an important gene of WSSV. |
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| Keywords: | White Spot Syndrome Virus(WSSV) p9 gene microarray immunofluorescent labeling |
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