| 血管抑素3A工程蛋白的柱复性与离子交换纯化 |
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| 引用本文： | 肖克,陆兵,夏杰,徐殿胜.血管抑素3A工程蛋白的柱复性与离子交换纯化[J].华东理工大学学报(自然科学版),2006,32(1):43-46. |
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| 作者姓名： | 肖克 陆兵 夏杰 徐殿胜 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237;华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 上海市-SK研究与发展基金 |
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| 摘 要： | 由大肠杆菌以包涵体形式表达的一种抗内皮细胞生长工程蛋白(A nti-ang iogen ic agent,简称3A)经变性,Sephacry l S-100 HR柱复性,SP Sepharose FF离子交换吸附纯化,SephadexG-25脱盐,获得复性率为53.47%,HPLC纯度为92.52%的3A活性蛋白。以猪髋动脉内皮细胞为受检细胞,表明纯化蛋白具有抑制内皮细胞生长的特性。
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| 关 键 词： | 内皮细胞生长抑素 3A蛋白 柱复性 离子交换吸附 |
| 文章编号： | 1006-3080（2006）01-0043-04 |
| 收稿时间： | 2004-12-11 |
| 修稿时间： | 2004年12月11 |
Separation of Anti-Angiogenic Agent from Inclusion Bodies by Column Refolding and Ion Exchange Adsorption |
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| XIAO Ke,LU Bing,XIA Jie,XU Dian-sheng.Separation of Anti-Angiogenic Agent from Inclusion Bodies by Column Refolding and Ion Exchange Adsorption[J].Journal of East China University of Science and Technology,2006,32(1):43-46. |
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| Authors: | XIAO Ke LU Bing XIA Jie XU Dian-sheng |
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| Abstract: | The isolation and purification of anti-angiogenic agent(3A),a new functional fragment found in the tPA was studied.This process was started with the denaturation of anti-angiogenic agent protein expressed and accumulated in the the form of inclusion bodies in the cell of Escherichia coli.After column refolding using Sephacryl S-100 HR,SP Sepharose FF ion exchange adsorption,and Sephadex G-25 desalting,the active protein recovery and the purity of 3A determined by HPLC is(53.47%) and(92.52%)(respectively).It was confirmed that the product protein can inhibit the growth of endothelioid cell. |
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| Keywords: | angiostatin 3A protein column refolding ion exchange adsorption |
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