Identification of differentially expressed genes in photo-period sensitive genie male sterile rice by randomly amplified cDNAs using RAPD primers

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation. Two populations were analyzed using cDNA-RAPD. The results showed that: ( i ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. ( ii ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( iii ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.