| 中华眼镜蛇毒组份Ⅲ的碘化标记物制备及质量鉴定 |
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| 引用本文： | 侯力强,强永刚,赵路宁,林振桃,廖永华,管锦霞.中华眼镜蛇毒组份Ⅲ的碘化标记物制备及质量鉴定[J].内蒙古民族大学学报(自然科学版),2004,19(2):203-205. |
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| 作者姓名： | 侯力强 强永刚 赵路宁 林振桃 廖永华 管锦霞 |
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| 作者单位： | 1. 广州医学院,蛇毒研究所,广东,广州,510182
2. 广州医学院,实验核医学教研室,广东,广州,510182 |
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| 摘 要： | 目的：研究对眼镜蛇毒(Naja naja atra venom)组份Ⅲ进行^125I放射性标记的方法及其质量评价．方法：用氯胺-T法碘化标记眼镜蛇毒组份Ⅲ．结果：用氯胺-T法按^125I标记组份Ⅲ，标记物放化纯为98．38％，比活度为0．945mCi／mg，RF值为0．76，每个组份Ⅲ分子上带0．56个^125I原子，与非标记组份Ⅲ有相同的生物学活性和理化性质．结论：所标记的眼镜蛇毒组份Ⅲ符合放射结合分析的要求．
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| 关 键 词： | 组份Ⅲ 眼镜蛇毒 ^125I放射标记 |
| 文章编号： | 1671-0185(2004)02-0203-03 |
| 修稿时间： | 0200年5月20日 |
Preparation and Quality Identification of Iodide of Fraction III Isolated from Naja naja atra Venom |
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| HOU Li-qiang,QIANG Yong-gang,ZHAO Lu-ning,LIN Zhen-tao,LIAO Yong-hua,GUAN Jin-xia.Preparation and Quality Identification of Iodide of Fraction III Isolated from Naja naja atra Venom[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2004,19(2):203-205. |
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| Authors: | HOU Li-qiang QIANG Yong-gang ZHAO Lu-ning LIN Zhen-tao LIAO Yong-hua GUAN Jin-xia |
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| Institution: | HOU Li-qiang~1,QIANG Yong-gang~2,ZHAO Lu-ning~1,LIN Zhen-tao~1,LIAO Yong-hua~2,GUAN Jin-xia~1 |
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| Abstract: | To study the methods with which Fraction III isolated from Naja naja atra venom was ()~~~~~~~~(125)I-labelled and quality evaluation. Methods:Fraction III from Naja naja atra venom was labelled with ()~~~~~~~~(125)I by chloramine-T method. Results:The marker's radiochemical purification was 98.38%, the ratio of activity was 0.945mCi/mg,and the RF was 0.76. Each molecule of Fraction III carried 0.56 ()~~~~~~~~(125)I atom, and their biological activity and physiochemical property equalled that of the unlabelled Fraction III. Conclusion:The ()~~~~~~~~(125)I- labelled Fraction III isolated from Naja naja atra venom was fitted to the demand of radiation combinant analyse. |
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| Keywords: | Fraction III Naja naja atra venom ()~(125)I-labelled |
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