Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis in<Emphasis Type="Italic">Taxus chinensis</Emphasis> suspension cells |
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Authors: | Maojun?Xu Jufang?Dong Email author" target="_blank">Muyuan?ZhuEmail author |
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Institution: | XU Mao jun;;DONG Jufang;;ZHU Muyuan College of Life Sciences, Zhejiang University, Hangzhou 310012, China College of Food Sciences and Biotechnology and Environmental Engineering, Hangzhou University of Commerce, Hangzhou 310035, China |
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Abstract: | Elicitor prepared from the cell walls of Peni-
cillium citrinum induces multiple responses of Taxus
chinensis cells, including nitric oxide (NO) generation, se-
quentially followed by the activation of PAL and synthesis of
taxol. NO scavenger cPITO and nitric oxide synthase (NOS)
inhibitor PBITU prevent the latter two reactions, all of which
are triggered in the absence of elicitor by NO donor sodium
nitroprusside (SNP). The elicitor-induced NO release of
Taxus chinensis suspension cells is strongly inhibited by
PBITU. Thesc results demonstrate a causal reationship be-
tween NO generation and the latter two reactions of Taxus
chinensis cells to the elicitor, and also indicate that NO, pro-
duced via NOS in Tasus chinensis cells treated with fungal
elicitor might act as an essential signaling molecule for trig-
gering the activation of PAL and synthesis of taxol. |
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Keywords: | nitric oxide elicitor PAL Taxus chinensis cell taxol |
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