| 血管内皮细胞分化实验模型的建立 |
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| 引用本文： | 李瑞,赵静,陈莉黎,王力,苗俊英.血管内皮细胞分化实验模型的建立[J].山东大学学报(理学版),2005,40(6):103-106. |
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| 作者姓名： | 李瑞 赵静 陈莉黎 王力 苗俊英 |
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| 作者单位： | 山东大学,生命科学学院发育研究所,山东,济南,250100;山东大学,生命科学学院发育研究所,山东,济南,250100;山东大学,生命科学学院发育研究所,山东,济南,250100;山东大学,生命科学学院发育研究所,山东,济南,250100;山东大学,生命科学学院发育研究所,山东,济南,250100 |
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| 摘 要： | 取X期的鸡胚盘细胞进行体外培养，细胞于24h后开始贴壁生长，72h后，开始向上皮类细胞分化．用不同浓度的成纤维细胞生长因子（FGF）处理刚分离的胚盘细胞，结合间接免疫荧光法检测细胞第Ⅷ凝血因子相关抗原的表达，初步发现大于100μg/L的FGF能诱导胚盘细胞向内皮细胞分化．
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| 关 键 词： | 胚盘细胞 血管内皮细胞 细胞分化 实验模型 |
| 文章编号： | 1671-9352（2005）06-0103-04 |
| 收稿时间： | 2005-09-07 |
| 修稿时间： | 2005年9月7日 |
Setting up of a test model for the endothelium cell differentiation |
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| LI Rui,ZHAO Jing,CHEN Li-li,WANG Li,MIAO Jun-ying.Setting up of a test model for the endothelium cell differentiation[J].Journal of Shandong University,2005,40(6):103-106. |
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| Authors: | LI Rui ZHAO Jing CHEN Li-li WANG Li MIAO Jun-ying |
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| Institution: | Institute of Development Biology, College of Life Science, Shandong Univ., Jinan 250100, Shandong, China |
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| Abstract: | Cultured the chicken blasoderm cells of stages X in vitro.The cells began to grow by anchoring the dish wall.After 72 hours of culturing,the cells began to differentiate into the epithelium like cells.The cells were treated with different doses of FGF just after the dissociation.Then in the blastoderm cells of culturing, the expression of Factor VIII associated antigen was examincd by the indirect immunofluorescence assay.Results show that more than 100ng/mL of the FGF can induce the dissociated blastoderm cells to differentiate into endothelium cells.Thus,the dissociated chicken blastderm cells provide a remarkably simple test model to investigate the mechanism of the endothelium differentiation. |
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| Keywords: | blastoderm cells vascular endothelium cell cell differentiation test model |
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