两种不同基质对体外培养成骨细胞促贴壁生长的影响

目的 比较多聚赖氨酸(PoLy lysine PLL)与鼠尾胶原对成骨细胞体外培养生长的影响。方法 分别采用PLL和鼠尾胶原铺制培养皿，然后接种成骨细胞。于24 h、48 h、72 h镜下观察细胞形态及占培养皿面积的百分比。结果 24 h PLL组，细胞贴壁呈均匀分布状，贴壁面积比为(30.6±12.9)％；鼠尾胶原组细胞贴壁呈团状，贴壁面积比为(42.5±15.7)％，两者比较差异显著(P ＜0.05)；48 h PLL组，细胞呈均匀分布生长，贴壁面积比为(43.8±14.5)％；鼠尾胶原组，细胞团相互连接，生长面积比为(61.3±17.1)％，差异极显著(P ＜0.01)；72 h PLL组细胞相互连接生长面积比为(56.3±15.4)％；鼠尾胶原组，细胞连成片， 生长面积比为(94.4±14.1)％，两者比较差异极显著(P＜0.01)。结论 对体外培养成骨细胞鼠尾胶原促贴壁生长比PLL更为适宜。

Comparisonof Two Different Material Affecting Osteablast on Sticking to the Wall and Growth

Objective To compare polylysine and collogen made by rat’s tail affecting on sticking to the wall of culture dishes in vitro.Methods We paved culture dish’s wall with polylysine and collagen made by rat’s tail,then to planted osteoblast.At 24 h 48h 72 h,we observed,under microscope,the cell’s morphous and area percentage which they stuck to the wall of culture dishes.Result In the polylysine group,at 24 h,48 h,72 h,osteoblast adherence displayed hypodispersion and adherence area ratio is 30.6%±12.9%,43.8%±14.5%,56.3%±15.4%,respectively.In the collagen made by rat’s tail group,at 24 h 48 h 72h,the cell adherence area ratio is 42.5%±15.7%,61.3%±17.1%,94.4%±14.1%,respectively.At 24 h,the cell adherence displayed mass;at 48 h,the cell displayed mass and conjunction each other;at 72 h,the cell displayed plaque.Two groups have significant difference.Conclusion The results showed that collagen made by rat’s tail is much suitable for osteoblast sticking wall growth.