凡纳滨对虾体内大黄苷的药代动力学特征

【目的】研究大黄苷(Rhein)在凡纳滨对虾体内的吸收、分布和代谢规律，并制定临床给药方案。【方法】以5 mg/kg剂量的大黄苷一次性肌肉注射凡纳滨对虾，利用高效液相色谱(HPLC)检测其在凡纳滨对虾血淋巴、肝胰脏、肌肉和鳃组织中的药物浓度-时间变化，并通过3P87软件进行数据处理，分析其在凡纳滨对虾体内的药代动力特征。【结果】大黄苷在4种组织里的达峰时间(Tmax)较早，分别为0.17 h、2.73 h、0.44 h、0.23 h；消除半衰期(T 1/2Ke)较短，分别为2.13 h、2.36 h、3.12 h、3.72 h；在房室模型选择上，大黄苷在4种组织中的最适药动模型均符合一级吸收一室模型。【结论】肌注大黄苷后，药物在凡纳滨对虾各组织中分布广泛、吸收快、清除能力强。大黄苷在凡纳滨对虾鳃部的浓度较高，这为利用其治疗凡纳滨对虾烂鳃病提供理论依据。

Pharmacokinetic Characteristics of Rhein in the Body of Penaeus vannamei

Objective]The absorption,distribution and metabolism of rhein in Penaeus vannamei were studied,and the clinical dosing regimen was established.Methods]We ana-lysed the pharmacokinetics of rhein in Penaeus vannamei,blood,hepatopancreas,muscle and gill were sampled after muscle inj ecting with a single dose of rhein (5 mg/kg),the contents of rhein was determined by HPLC,and 3P87 software were used to analyse the data process-ing.Results]Rhein in four kinds of tissue in the peak time (Tmax)early and were 0.17 h,2. 73 h,0.44 h,0.23 h;elimination half-life (T 1/2Ke)short were 2.13 h,2.36 h,3.12 h,3.72 h;in compartment model choice,the four organizations rhein optimum pharmacokinetic model are suitable for a one-compartment model.Conclusion]Rhein after intramuscular inj ection, the drug vannamei tissues peak time is short,wide distribution,rapid absorption,strong scav-enging ability.The concentration of rhein vannamei gills is high,which provides a theoretical basis for using it to treat gill disease of Penaeus vannamei.