杏鲍菇菌株的rDNA ITS序列鉴定和同工酶分析

【目的】鉴于诱变处理后杏鲍菇菌株在栽培性状上的明显差异,对两株杏鲍菇菌株(杏A和杏B)进行分子水平上的鉴定,分析两者的差异性。【方法】对杏鲍菇菌丝体样本的rDNA基因内转录间ITS片段进行PCR扩增并测序验证;同时应用同工酶电泳对菌丝体的酯酶、过氧化物酶和超氧化物歧化酶进行分析。【结果】通过GenBank数据库搜索和序列比对分析,两株菌株ITS序列相似度达到99%;菌丝体的酯酶、过氧化物酶和超氧化物歧化酶同工酶电泳检测无差异。【结论】杏A和杏B菌株为同一菌株,在分子水平上差异不明显。

Analysis on Isozyme and rDNA ITS Sequence of Pleurotus eryngii Strains

Objective]In view of the fact that the mutation of Pleurotus eryngii strains is dif-ferent in the cultivated character.Two strains of Pleurotus eryngii strains (A and B)are i-dentified at molecular level and analyze their difference.Methods]The rDNA fragment of Pleurotus eryngii mycelium samples was amplified by PCR and sequencing.Mycelium of es-terase,peroxidase and superoxide dismutase enzyme are analyzed by isozyme electrophore-sis.Results]Through the analysis of GenBank database search and sequence alignment,the similarity of ITS sequences of the two strains was reached to 9 9%;There was no difference between esterase,peroxidase and superoxide dismutase isozymes in the mycelium.Conclu-sion]A and B strain is the same strain,at the molecular level with insignificant difference.