响应面法优化狭鳕鱼鱼皮酸溶性胶原蛋白质的提取工艺

【目的】优化狭鳕鱼(Theragra chalcogramma)鱼皮中酸溶性胶原蛋白质的提取工艺,为实际生产提供工艺数据和理论依据。【方法】在乙酸浓度、提取时间和料液比的单因素实验基础上,以酸溶性胶原蛋白质提取率为响应值,采用Box-Behnken设计实验,建立提取率的二次多项式回归模型,考察3个因素对提取率的影响。【结果】对酸溶性胶原蛋白质提取率的影响为料液比提取时间乙酸浓度;交互项中料液比-提取时间项对提取率影响极其显著,料液比-乙酸浓度项对提取率影响显著,其他交互项对提取率影响不显著;通过实验数据结合回归模型进行数学分析,得到提取狭鳕鱼鱼皮酸溶性胶原蛋白质的最佳工艺参数:乙酸浓度0.5mol/L、提取时间40min、料液比1∶9。在优化参数条件下,酸溶性胶原蛋白质提取率的理论预测值为20.53%,实测值为20.10%;试验提取得到的胶原蛋白电泳图谱较为清晰,无杂条带出现。【结论】该回归模型合理可行,提取的产物基本保持了胶原蛋白质的原有结构。

Optimization of Extraction Process of Acid-soluble Collagen from the Walleye Pollock(Theragra chalcogramma) by Respone Surface Methodolgy

[Objective]Walleye pollock (Theragra chalcogramma )is one of the commercial fish species in China.The skin of pollock is usually dumped without utilization,which would cause environment pollution and resource waste.The main component of pollock skin is col-lagen.For effective use of the dumped skin to prepare collagen,it is necessary to obtain fuda-mental information about the pollock skin collagen.[Methods]In this research,on the basis of one-factor-at-a-time experiments,a Box-behnken design experiment was used to evaluate the optimal extraction conditions of acid-soluble collagen from the walleye pollock skin.The con-centration of acid,solid-to-liquid ratio and extraction time were the three test factors and the extraction rate of collagen was the target parameter.Response surface methodology was used to describe the influence of the three factors on the extraction rate of collagen and aquadratic polynomial regression model was developed to optimize the extraction conditions.[Results]The order of the factors influence was:Solid-to-liquid ratio > extraction time> concentration of acid. The interaction between the solid-to-liquid ratio and extraction time had a extremely significant effect on the collagen extraction rate,interaction between the solid-to-liquid ratio and concentra-tion of acid had a significant effect on the collagen extraction rate,while other interactions had no significant effect.Through the experiment combined with regression model,the spe-cific parameters were:The concentration of acid was 0.5 mol/L,the extraction time was 40 min,and the solid-to-liquid ratio was 1:9 .Under these conditions,the extraction rate was 20.10%,which was similar to the theoretical value (20.53%)predicted by theregression model.The SDS-PAGE pattern was clear.[Conclusion]The regression model was reasonable and feasible.The collagen was of good purity and maintained its original structure.